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Literature summary extracted from

  • Scocca, J.J.
    Purification and substrate specificity of pyrimidine nucleoside phosphorylase from Haemophilus influenzae (1971), J. Biol. Chem., 246, 6606-6610.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.2.3 use of dithiothreitol and glycerol is necessary for stabilization during purification Haemophilus influenzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.2 uridine above 15 mM, competitive inhibition of cleavage of 5-bromodeoxyuridine Haemophilus influenzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.2 0.03
-
 5-Bromouridine
-
 Haemophilus influenzae
2.4.2.2 0.07
-
 5-methyluridine
-
 Haemophilus influenzae
2.4.2.2 0.1
-
 5-bromodeoxyuridine
-
 Haemophilus influenzae
2.4.2.2 0.11
-
 thymidine
-
 Haemophilus influenzae
2.4.2.2 0.13
-
 deoxyuridine
-
 Haemophilus influenzae
2.4.2.2 0.24
-
 uridine
-
 Haemophilus influenzae
2.4.2.2 0.38
-
 phosphate reaction with uridine Haemophilus influenzae
2.4.2.3 0.03
-
 5-Bromouridine
-
 Haemophilus influenzae
2.4.2.3 0.07
-
 5-methyluridine
-
 Haemophilus influenzae
2.4.2.3 0.1
-
 5-bromodeoxyuridine
-
 Haemophilus influenzae
2.4.2.3 0.11
-
 thymidine
-
 Haemophilus influenzae
2.4.2.3 0.13
-
 2'-deoxyuridine
-
 Haemophilus influenzae
2.4.2.3 0.24
-
 uridine
-
 Haemophilus influenzae

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.2 Haemophilus influenzae
-
-
-
2.4.2.3 Haemophilus influenzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.2
-
 Haemophilus influenzae
2.4.2.3 400fold Haemophilus influenzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.2 additional information
-
-
 Haemophilus influenzae
2.4.2.3 510
-
-
 Haemophilus influenzae

Storage Stability

EC Number Storage Stability Organism
2.4.2.2 -20°C, in presence of phosphate, glycerol and dithiothreitol, stable for 6 months Haemophilus influenzae
2.4.2.3 -20°C, stable for 6 months Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.2 5-bromodeoxyuridine + phosphate
-
 Haemophilus influenzae 5-bromouracil + deoxyribose 1-phosphate
-
 ?
2.4.2.2 5-bromouridine + phosphate
-
 Haemophilus influenzae 5-bromouracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 5-methyluridine + phosphate
-
 Haemophilus influenzae 5-methyluracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 cytidine + phosphate no activity Haemophilus influenzae cytosine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 deoxyuridine + phosphate
-
 Haemophilus influenzae uracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 thymidine + phosphate
-
 Haemophilus influenzae thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate
-
 Haemophilus influenzae uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 2'-deoxyuridine + phosphate 12% of the activity with uridine Haemophilus influenzae uracil + deoxyribose 1-phosphate
-
 ?
2.4.2.3 5-bromo-2'-deoxyuridine + phosphate 75% of the activity with uridine Haemophilus influenzae 5-bromouracil + 2-deoxribose 1-phosphate
-
 ?
2.4.2.3 5-bromouracil + deoxyribose 1-phosphate
-
 Haemophilus influenzae 5-bromodeoxyuridine + phosphate
-
 ?
2.4.2.3 5-bromouridine + phosphate 40% of the activity with uridine Haemophilus influenzae 5-bromouracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 5-methyluridine + phosphate 27% of the activity with uridine Haemophilus influenzae 5-methyluracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 additional information no substrate: cytidine Haemophilus influenzae ?
-
 ?
2.4.2.3 thymidine + phosphate 21% of the activity with uridine Haemophilus influenzae thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 uracil arabinoside + phosphate 10% of the activity with uridine Haemophilus influenzae uracil + arabinose-1-phosphate
-
 ?
2.4.2.3 uridine + phosphate
-
 Haemophilus influenzae uracil + alpha-D-ribose 1-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.2 6.9
-
 deoxynucleoside substrates Haemophilus influenzae
2.4.2.2 7.4
-
 ribonucleosides and uracil arabinoside Haemophilus influenzae
2.4.2.3 6.9
-
 deoxynucleoside phosphorolysis Haemophilus influenzae
2.4.2.3 7.4
-
 phosphorolysis of ribonucleosides and uracil arabinoside Haemophilus influenzae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.2 0.25
-
 uridine
-
 Haemophilus influenzae