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Literature summary extracted from

  • Loke, P.; Goh, L.L.; Soh, B.S.; Yeow, P.; Sim, T.S.
    Purification and characterization of recombinant malate synthase enzymes from Streptomyces coelicolor A3(2) and S. clavuligerus NRRL3585 (2002), J. Ind. Microbiol. Biotechnol., 28, 239-243.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.9 expression in Escherichia coli Streptomyces coelicolor
2.3.3.9 expression in Escherichia coli Streptomyces clavuligerus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.9 acetyl-CoA slight reduction of enzyme activity at high concentrations of acety-CoA Streptomyces clavuligerus
2.3.3.9 acetyl-CoA slight reduction of enzyme activity at high concentrations of acety-CoA Streptomyces coelicolor
2.3.3.9 glyoxylate slight reduction of enzyme activity at high concentrations of glyoxylate Streptomyces clavuligerus
2.3.3.9 glyoxylate slight reduction of enzyme activity at high concentrations of glyoxylate Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.9 additional information
-
 additional information
-
 Streptomyces coelicolor
2.3.3.9 additional information
-
 additional information
-
 Streptomyces clavuligerus
2.3.3.9 0.00059
-
 glyoxylate
-
 Streptomyces clavuligerus
2.3.3.9 0.00349
-
 glyoxylate
-
 Streptomyces coelicolor

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.9 Mg2+ required Streptomyces coelicolor
2.3.3.9 Mg2+ required Streptomyces clavuligerus
2.3.3.9 Mg2+ optimal concentration 0.01 mM MgCl2 Streptomyces clavuligerus
2.3.3.9 Mg2+ optimal concentration 0.005 mM MgCl2 Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.9 Streptomyces clavuligerus Q9ZH77 NRRL3585
-
2.3.3.9 Streptomyces clavuligerus NRRL 3585 Q9ZH77 NRRL3585
-
2.3.3.9 Streptomyces coelicolor Q9RKU9 M130
-
2.3.3.9 Streptomyces coelicolor A3(2) Q9RKU9 M130
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.9 recombinant enzymes Streptomyces coelicolor
2.3.3.9 recombinant enzymes Streptomyces clavuligerus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.9 0.0012
-
-
 Streptomyces clavuligerus
2.3.3.9 0.0262
-
-
 Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.9 glyoxylate + acetyl-CoA + H2O the optimum concentration of acetyl-CoA 0.0001 mM, the optimum concentration of glyoxylate 0.001 mM Streptomyces coelicolor (S)-malate + CoA
-
 ?
2.3.3.9 glyoxylate + acetyl-CoA + H2O the optimum concentration of acetyl-CoA 0.0001 mM, the optimum concentration of glyoxylate 0.001 mM Streptomyces clavuligerus (S)-malate + CoA
-
 ?
2.3.3.9 glyoxylate + acetyl-CoA + H2O the optimum concentration of acetyl-CoA 0.0001 mM, the optimum concentration of glyoxylate 0.001 mM Streptomyces coelicolor A3(2) (S)-malate + CoA
-
 ?
2.3.3.9 glyoxylate + acetyl-CoA + H2O the optimum concentration of acetyl-CoA 0.0001 mM, the optimum concentration of glyoxylate 0.001 mM Streptomyces clavuligerus NRRL 3585 (S)-malate + CoA
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.3.9 20 45
-
 Streptomyces coelicolor
2.3.3.9 20 45
-
 Streptomyces clavuligerus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.3.9 8 10 significant reduction of specific activity at pH 7.0 and below Streptomyces coelicolor
2.3.3.9 8 10 significant reduction of specific activity at pH 7.0 and below Streptomyces clavuligerus