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Literature summary extracted from

  • Williams, D.; Schachter, H.
    Mucin synthesis. I. Detection in canine submaxillary glands of an N-acetylglucosaminyltransferase which acts on mucin substrates (1980), J. Biol. Chem., 255, 11247-11252.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.102 Empigen BB activation, detergent Canis lupus familiaris
2.4.1.102 Triton X-100 4fold stimulation at 0.125% v/v Canis lupus familiaris

General Stability

EC Number General Stability Organism
2.4.1.102 freeze-thawing, after 2 cycles 13% loss of activity Canis lupus familiaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.102 Ca2+ slight inhibition Canis lupus familiaris
2.4.1.102 EDTA slight inhibition Canis lupus familiaris
2.4.1.102 Mn2+ slight stimulation at 5 mM, inhibition at higher concentrations Canis lupus familiaris
2.4.1.102 Zn2+ strong inhibition Canis lupus familiaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.102 1
-
 UDP-N-acetyl-D-glucosamine
-
 Canis lupus familiaris
2.4.1.102 5.2
-
 asialo-blood group A negative PSM
-
 Canis lupus familiaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.102 microsome
-
 Canis lupus familiaris
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.102 Mg2+ slight stimulation, 5 mM Canis lupus familiaris
2.4.1.102 Mn2+ slight stimulation, 5 mM, inhibits at higher concentration Canis lupus familiaris

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.102 Canis lupus familiaris
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.102 submaxillary gland
-
 Canis lupus familiaris
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.102 frozen in liquid nitrogen, several months Canis lupus familiaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.102 UDP-N-acetyl-D-glucosamine + asialo-alpha1 acid glycoprotein
-
 Canis lupus familiaris ?
-
 ?
2.4.1.102 UDP-N-acetyl-D-glucosamine + asialo-blood group A negative PSM acceptor contains no fucose Canis lupus familiaris ?
-
 ?
2.4.1.102 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-benzyl
-
 Canis lupus familiaris UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-benzyl
-
 ?
2.4.1.102 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-phenyl
-
 Canis lupus familiaris UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-phenyl
-
 ?
2.4.1.102 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R
-
 Canis lupus familiaris UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R i.e. core class 2 ?
2.4.1.102 UDP-N-acetyl-D-glucosamine + submaxillary mucin polypeptide porcine or ovine origin of acceptor substrate Canis lupus familiaris ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.102 25
-
 assay at Canis lupus familiaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.102 25
-
 25°C, 2 h Canis lupus familiaris
2.4.1.102 37
-
 unstable Canis lupus familiaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.102 7
-
-
 Canis lupus familiaris