Literature summary extracted from

Cardinaud, R.
Nucleoside deoxyribosyltransferase from Lactobacillus helveticus (1978), Methods Enzymol., 51, 446-455.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.4.2.6	adenine	-	Lactobacillus helveticus
2.4.2.6	cytosine	-	Lactobacillus helveticus
2.4.2.6	deoxyadenosine	-	Lactobacillus helveticus
2.4.2.6	deoxycytidine	-	Lactobacillus helveticus
2.4.2.6	deoxyinosine	-	Lactobacillus helveticus
2.4.2.6	hypoxanthine	-	Lactobacillus helveticus
2.4.2.6	Tris	complete inhibition of deoxyribosyltransferase I at 0.1 M, pH 6.9. 62% inhibition of nucleoside deoxyribosyltransferase I at 0.8 M, pH 5.9	Lactobacillus helveticus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.4.2.6	0.019	-	adenine	reaction with deoxycytidine	Lactobacillus helveticus
2.4.2.6	0.041	-	deoxyinosine	-	Lactobacillus helveticus
2.4.2.6	0.086	-	hypoxanthine	-	Lactobacillus helveticus
2.4.2.6	0.09	-	deoxycytidine	-	Lactobacillus helveticus
2.4.2.6	0.12	-	deoxyadenosine	-	Lactobacillus helveticus
2.4.2.6	0.22	-	cytosine	-	Lactobacillus helveticus
2.4.2.6	0.35	-	deoxyinosine	-	Lactobacillus helveticus
2.4.2.6	0.45	-	deoxyadenosine	-	Lactobacillus helveticus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.4.2.6	86000	-	-	Lactobacillus helveticus

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.4.2.6	Lactobacillus helveticus	-	nucleoside deoxyribosyltransferase-I and nucleoside deoxyribosyltransferase-I	-

Reaction

EC Number	Reaction	Comment	Organism	Reaction ID
2.4.2.6	2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1	ping-pong bi bi mechanism	Lactobacillus helveticus	a

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.4.2.6	5'-fluoro-5'-thymidine + adenine	deoxyribosyltransferase II	Lactobacillus helveticus	5'-fluoro-5'-deoxythymine + adenosine	-	?
2.4.2.6	deoxycytidine + adenine	-	Lactobacillus helveticus	cytosine + deoxyadenosine	-	r
2.4.2.6	deoxyinosine + adenine	-	Lactobacillus helveticus	hypoxanthine + deoxyadenosine	-	r
2.4.2.6	additional information	nucleoside deoxyribosyltransferase I is strictly specific for transfer between purine bases. Nucleoside deoxyribosyltransferase II catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine	Lactobacillus helveticus	?	-	?
2.4.2.6	thymidine + xanthine	-	Lactobacillus helveticus	9-deoxyribosylxanthine + 7-deoxyribosylxanthine	20% 9-deoxyribosylxanthine + 80% 7-deoxyribosylxanthine	?

Synonyms

EC Number	Synonyms	Comment	Organism
2.4.2.6	nucleoside deoxyribosyltransferase I	purine nucleoside:purine deoxyribosyltransferase:strictly specific for transfer between purine bases	Lactobacillus helveticus
2.4.2.6	nucleoside deoxyribosyltransferase II	purine(pyrimidine) nucleoside:purine(pyrimidine) deoxyribosyltransferase:catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine	Lactobacillus helveticus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.4.2.6	5.8	-	-	Lactobacillus helveticus

pH Stability

EC Number	pH Stability	pH Stability Maximum	Comment	Organism
2.4.2.6	6.5	-	optimal stability	Lactobacillus helveticus

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
2.4.2.6	0.021	-	adenine	-	Lactobacillus helveticus
2.4.2.6	0.039	-	adenine	-	Lactobacillus helveticus
2.4.2.6	0.079	-	hypoxanthine	-	Lactobacillus helveticus
2.4.2.6	0.092	-	deoxyadenosine	-	Lactobacillus helveticus
2.4.2.6	0.095	-	deoxycytidine	-	Lactobacillus helveticus
2.4.2.6	0.17	-	cytosine	-	Lactobacillus helveticus
2.4.2.6	0.34	-	deoxyinosine	-	Lactobacillus helveticus
2.4.2.6	0.43	-	deoxyadenosine	-	Lactobacillus helveticus

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Release 2023.1 (January 2023)