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Literature summary extracted from

  • Kainou, T.; Okada, K.; Suzuki, K.; Nakagawa, T.; Matsuda, H.; Kawamukai, M.
    Dimer formation of octaprenyl-diphosphate synthase (IspB) is essential for chain length determination of ubiquinone (2001), J. Biol. Chem., 276, 7876-7883.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.90 expression in Escherichia coli and Saccharomyces cerevisiae Escherichia coli K-12

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.90 A79Y no activity. Cells harboring wild-type ispB and the A79Y mutant produce mainly ubiquinone-6, although the activity of the enzyme with the A79Y mutation is completely abolished. Although the A79Y mutant is functionally inactive, it can regulate activity upon forming a heterodimer with wild-type IspB, and this dimer formation is important for the determination of the isoprenoid chain length Escherichia coli K-12
2.5.1.90 F75A resulting ubiquinone species are almost the same as those produced by the wild-type enzyme Escherichia coli K-12
2.5.1.90 I32V resulting ubiquinone species are almost the same as those produced by the wild-type enzyme Escherichia coli K-12
2.5.1.90 K170A mutant cannot be isolated, mutant protein does not retain functional activity Escherichia coli K-12
2.5.1.90 K170G mutant cannot be isolated, mutant protein does not retain functional activity Escherichia coli K-12
2.5.1.90 K235L main product is ubiquinone-8 Escherichia coli K-12
2.5.1.90 L31V resulting ubiquinone species are almost the same as those produced by the wild-type enzyme Escherichia coli K-12
2.5.1.90 R321A normal growth at 30°C, no growth at 43°C, main product is ubiquinone-8 Escherichia coli K-12
2.5.1.90 R321D normal growth at 30°C, no growth at 43°C Escherichia coli K-12
2.5.1.90 R321V products are ubiquinone-6 and ubiquinone-7, slow growth at 43°C Escherichia coli K-12
2.5.1.90 Y37A mutant cannot be isolated, mutant protein does not retain functional activity Escherichia coli K-12
2.5.1.90 Y37A/Y38A increased production of ubiquinone-6 Escherichia coli K-12
2.5.1.90 Y38A products are ubiquinone-6 and ubiquinone-7 Escherichia coli K-12
2.5.1.90 Y38A/R321V products are ubiquinone-5 and ubiquinone-6 Escherichia coli K-12
2.5.1.90 Y61V resulting ubiquinone species are almost the same as those produced by the wild-type enzyme Escherichia coli K-12

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.90 Escherichia coli K-12 P0AD57
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.90 additional information residues Tyr-38, Lys-235, and Arg-321 are involved in chain length determination Escherichia coli K-12 ?
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Synonyms

EC Number Synonyms Comment Organism
2.5.1.90 octaprenyl-diphosphate synthase
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