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Literature summary extracted from

  • Nidetzky, B.; Eis, C.; Albert, M.
    Role of non-covalent enzyme-substrate interactions in the reaction catalysed by cellobiose phosphorylase from Cellulomonas uda (2000), Biochem. J., 351, 649-659.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.20 4-deoxy-D-glucose competitive with D-glucose, dead end inhibitor Cellulomonas uda
2.4.1.20 4-thio-cellobiose weak inhibition in both directions of reaction Cellulomonas uda
2.4.1.20 cellobiose substrate inhibition, competitive with glucose-1-phosphate Cellulomonas uda
2.4.1.20 D-glucose mixed type inhibition against cellobiose, pure beta-D-glucose inhibits stronger than mixture of both anomers Cellulomonas uda
2.4.1.20 D-glucose 1-phosphate substrate inhibition, competitive with cellobiose Cellulomonas uda

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.20 1.2
-
 phosphate
-
 Cellulomonas uda
2.4.1.20 2.15
-
 alpha-D-glucose 1-phosphate
-
 Cellulomonas uda
2.4.1.20 2.26
-
 D-glucose
-
 Cellulomonas uda
2.4.1.20 4.3
-
 cellobiose
-
 Cellulomonas uda
2.4.1.20 7.4
-
 6-deoxy-6-fluoro-D-glucose
-
 Cellulomonas uda
2.4.1.20 10.2
-
 6-deoxy-D-glucose
-
 Cellulomonas uda
2.4.1.20 10.8
-
 D-glucoseamine
-
 Cellulomonas uda
2.4.1.20 15.9
-
 D-xylose
-
 Cellulomonas uda
2.4.1.20 17.2
-
 2-deoxy-2-fluoro-D-glucose
-
 Cellulomonas uda
2.4.1.20 24.3
-
 2-deoxy-D-glucose
-
 Cellulomonas uda
2.4.1.20 27.3
-
 D-mannose
-
 Cellulomonas uda

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.20 cellobiose + phosphate Cellulomonas uda important for energy efficient catabolism of cellobiose in cytoplasm alpha-D-glucose 1-phosphate + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.20 Cellulomonas uda
-
-
-
2.4.1.20 Cellulomonas uda
-
 DSM 20108
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.20 alpha-D -glucose 1-fluoride + D-glucose
-
 Cellulomonas uda cellobiose + fluoride
-
 ir
2.4.1.20 alpha-D-glucose 1-phosphate + 2-deoxy-2-fluoro-D-glucose
-
 Cellulomonas uda ?
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
-
 Cellulomonas uda ?
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + 6-deoxy-6-fluoro-D-glucose
-
 Cellulomonas uda ?
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
-
 Cellulomonas uda ?
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + D-glucosamine
-
 Cellulomonas uda ?
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + D-glucose
-
 Cellulomonas uda cellobiose + phosphate
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + D-mannose
-
 Cellulomonas uda ?
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + D-xylose
-
 Cellulomonas uda ?
-
 ?
2.4.1.20 beta-D-glucose 1-phosphate + alpha-D-glucopyranosyl fluoride beta-D-glucopyranosyl fluoride is not ulilized Cellulomonas uda beta-cellobiose + HF
-
 ?
2.4.1.20 cellobiose + arsenate
-
 Cellulomonas uda D-glucose + alpha-D-glucose 1-arsenate alpha-D-glucose 1-arsenate hydrolyses spontaneously to form D-glucose and arsenate ir
2.4.1.20 cellobiose + phosphate beta-cellobiose preferred over alpha-anomer Cellulomonas uda alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.20 cellobiose + phosphate ternary-complex kinetic mechanism Cellulomonas uda alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.20 cellobiose + phosphate important for energy efficient catabolism of cellobiose in cytoplasm Cellulomonas uda alpha-D-glucose 1-phosphate + D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.20 CuCbP
-
 Cellulomonas uda

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.20 5.6
-
 D-glucoseamine
-
 Cellulomonas uda
2.4.1.20 6.6
-
 D-mannose
-
 Cellulomonas uda
2.4.1.20 8
-
 2-deoxy-2-fluoro-D-glucose
-
 Cellulomonas uda
2.4.1.20 8.7
-
 D-xylose
-
 Cellulomonas uda
2.4.1.20 11.6
-
 2-deoxy-D-glucose
-
 Cellulomonas uda
2.4.1.20 29.1
-
 cellobiose
-
 Cellulomonas uda
2.4.1.20 43.2
-
 D-glucose
-
 Cellulomonas uda
2.4.1.20 44.6
-
 6-deoxy-6-fluoro-D-glucose
-
 Cellulomonas uda
2.4.1.20 55.3
-
 6-deoxy-D-glucose
-
 Cellulomonas uda

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.20 2.2
-
 D-glucose
-
 Cellulomonas uda
2.4.1.20 3.2
-
 D-glucose 1-phosphate
-
 Cellulomonas uda
2.4.1.20 4.4
-
 4-deoxy-D-glucose
-
 Cellulomonas uda
2.4.1.20 13
-
 4-thio-cellobiose in presence of saturating concentrations of phosphate Cellulomonas uda
2.4.1.20 14.8
-
 cellobiose
-
 Cellulomonas uda