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Literature summary extracted from

  • Kearns, A.E.; Campbell, S.C.; Westley, J.; Schwartz, N.B.
    Initiation of chondroitin sulfate biosynthesis: a kinetic analysis of UDP-D-xylose: core protein beta-D-xylosyltransferase (1991), Biochemistry, 30, 7477-7483.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.26 additional information competitive inhibition of the acceptor substrates Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.26 additional information
-
 additional information
-
 Rattus norvegicus
2.4.2.26 0.00011
-
 HF-degraded chondroitin sulfate proteoglycan
-
 Rattus norvegicus
2.4.2.26 0.062
-
 CDEASGIGPEVPDDRD
-
 Rattus norvegicus
2.4.2.26 0.062
-
 CDEASGIGPDDRD
-
 Rattus norvegicus
2.4.2.26 0.11
-
 cartilage chondroitin sulfate proteoglycan
-
 Rattus norvegicus
2.4.2.26 0.12
-
 FMLEDEASGIGP
-
 Rattus norvegicus
2.4.2.26 0.18
-
 UDP-D-xylose
-
 Rattus norvegicus
2.4.2.26 0.18
-
 UDP-D-xylose cosubstrate: CDEASGIGPDDRD Rattus norvegicus
2.4.2.26 0.79
-
 SDDYSGSGSG
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.26 UDP-alpha-D-xylose + [protein]-L-serine Rattus norvegicus
-
 UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.26 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.26 UDP-alpha-D-xylose + [protein]-L-serine = UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine most probable mechanism: ordered single displacement with UDPxylose as the leading substrate and the xylosylated peptide as the first product released Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.26 chondrosarcoma cell
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.26 UDP-alpha-D-xylose + [protein]-L-serine
-
 Rattus norvegicus UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
-
 ?
2.4.2.26 UDP-alpha-D-xylose + [protein]-L-serine transfers a beta-D-xylosyl residue from UDP-D-xylose to the serine hydroxyl group of an acceptor protein substrate Rattus norvegicus UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
-
 ?
2.4.2.26 UDP-D-xylose + cartilage chondroitin sulfate proteoglycan HF-deglycosylated acceptor Rattus norvegicus UDP + cartilage chondroitin sulfate proteoglycan with xylosylserine
-
 ?
2.4.2.26 UDP-D-xylose + cartilage chondroitin sulfate proteoglycan the consensus sequence, acidic-acidic-Xxx-Ser-Gly-Xxx-Gly, in the acceptor proteoglycan is important Rattus norvegicus UDP + cartilage chondroitin sulfate proteoglycan with xylosylserine
-
 ?
2.4.2.26 UDP-D-xylose + cartilage chondroitin sulfate proteoglycan deglycosylation by Staphylococcus aureus V8 protease decreases the activity with the resulting peptides Rattus norvegicus UDP + cartilage chondroitin sulfate proteoglycan with xylosylserine
-
 ?
2.4.2.26 UDP-D-xylose + CDEASGIGPDDRD
-
 Rattus norvegicus UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + CDEASGIGPEVPDDRD synthetic peptide Rattus norvegicus UDP + CDEAS(-D-xylose)GIGPEVPDDRD
-
 ?
2.4.2.26 UDP-D-xylose + FMLEDEASGIGP synthetic peptide Rattus norvegicus UDP + FMLEDEAS(-D-xylose)GIGP
-
 ?
2.4.2.26 UDP-D-xylose + HF-degraded chondroitin sulfate proteoglycan
-
 Rattus norvegicus UDP + ?
-
 ?
2.4.2.26 UDP-D-xylose + SDDYSGSGSG synthetic peptide Rattus norvegicus UDP + SDDYS(-D-xylose)GSGSG
-
 ?
2.4.2.26 UDP-D-xylose + SDDYSGSGSG
-
 Rattus norvegicus UDP + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.26 UDP-D-xylose:core protein beta-D-xylosyltransferase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.26 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.26 6.5
-
 assay at Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.26 additional information
-
 additional information competitive inhibition of the acceptor substrates Rattus norvegicus