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Literature summary extracted from

  • Kim, H.G.; Yang, S.M.; Lee, Y.C.; Do, S.I.; Chung, I.S.; Yang, J.M.
    High-level expression of human glycosyltransferases in insect cells as biochemically active form (2003), Biochem. Biophys. Res. Commun., 305, 488-493.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.1 high level functional expression in Spodoptera frugiperda Sf9 insect cells via baculovirus infection using a vector that fuses the enzyme to the mouse IgM signal peptide and the IgG binding domain of the Staphylococcus aureus protein A at the N-terminus for secretion of the recombinant protein into the culture medium Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.1 0.38
-
 CMP-N-acetylneuraminate
-
 Rattus norvegicus
2.4.3.1 0.555
-
 CMP-N-acetylneuraminate recombinant enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Homo sapiens
-
-
-
2.4.3.1 Rattus norvegicus
-
 commercial product
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.1 recombinant from cell culture medium after expression in insect cells Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.1 commercial preparation
-
 Rattus norvegicus
-
2.4.3.1 liver
-
 Homo sapiens
-
2.4.3.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.3.1 1.6
-
 purified, recombinant from Sf9 insect cells Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + asialo-fetuin
-
 Homo sapiens CMP + fetuin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-fetuin
-
 Rattus norvegicus CMP + fetuin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Homo sapiens CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.3.1 37
-
 assay at Homo sapiens
2.4.3.1 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.3.1 6.5
-
 assay at Homo sapiens
2.4.3.1 6.5
-
 assay at Rattus norvegicus