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Literature summary extracted from

  • Munch-Petersen, B.; Piskur, J.; Sondergaard, L.
    The single deoxynucleoside kinase in Drosophila melanogaster, Dm-dNK, is multifunctional and differs from the mammalian deoxynucleoside kinases (1998), Adv. Exp. Med. Biol., 431, 465-469.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.145 2'-deoxycytidine competitive with thymidine Drosophila melanogaster
2.7.1.145 2'-deoxyguanosine
-
 Drosophila melanogaster
2.7.1.145 thymidine competitive with 2'-deoxycytidine Drosophila melanogaster
2.7.1.145 TTP feed-back inhibition Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.145 0.001
-
 thymidine pH 8.0, 22°C Drosophila melanogaster
2.7.1.145 0.001
-
 2'-deoxycytidine pH 8.0, 22°C Drosophila melanogaster
2.7.1.145 0.0014
-
 ATP pH 8.0, 22°C, reaction with 2'-deoxycytidine Drosophila melanogaster
2.7.1.145 0.0022
-
 ATP pH 8.0, 22°C, reaction with thymidine Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.145 29000
-
 1 * 29000, SDS-PAGE Drosophila melanogaster
2.7.1.145 30000
-
 gel filtration Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.145 Drosophila melanogaster
-
 S-2
-
2.7.1.145 Drosophila melanogaster S-2
-
 S-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.145
-
 Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.145 embryo
-
 Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.145 30
-
-
 Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.145 1-beta-D-arabinofuranosylcytosine + ATP 50% of the activity with thymidine and 2'-deoxycytidine Drosophila melanogaster 1-beta-D-arabinofuranosylcytosine 5'-phosphate + ADP
-
 ?
2.7.1.145 1-beta-D-arabinofuranosylthymine + ATP 15% of the activity with thymidine and 2'-deoxycytidine Drosophila melanogaster 1-beta-D-arabinofuranosylthymine 5'-phosphate + ADP
-
 ?
2.7.1.145 2'-deoxyadenosine + ATP 15% of the activity with thymidine and 2'-deoxycytidine Drosophila melanogaster 2'-deoxyadenosine 5'-phosphate + ADP
-
 ?
2.7.1.145 2'-deoxycytidine + ATP
-
 Drosophila melanogaster 2'-deoxycytidine 5'-phosphate + ADP
-
 ?
2.7.1.145 2'-deoxycytidine + ATP
-
 Drosophila melanogaster S-2 2'-deoxycytidine 5'-phosphate + ADP
-
 ?
2.7.1.145 2'-deoxyguanosine + ATP 4% of the activity with thymidine and 2'-deoxycytidine Drosophila melanogaster 2'-deoxyguanosine 5'-phosphate + ADP
-
 ?
2.7.1.145 3'-azidothymidine + ATP 0.3% of the activity with thymidine and 2'-deoxycytidine Drosophila melanogaster 3'-azidothymidine 5'-phosphate + ADP
-
 ?
2.7.1.145 additional information no activity with acyclovir Drosophila melanogaster ?
-
 ?
2.7.1.145 additional information no activity with TMP Drosophila melanogaster ?
-
 ?
2.7.1.145 additional information CTP has the same phosphotransfer capability as ATP Drosophila melanogaster ?
-
 ?
2.7.1.145 additional information no activity with acyclovir Drosophila melanogaster S-2 ?
-
 ?
2.7.1.145 additional information no activity with TMP Drosophila melanogaster S-2 ?
-
 ?
2.7.1.145 additional information CTP has the same phosphotransfer capability as ATP Drosophila melanogaster S-2 ?
-
 ?
2.7.1.145 thymidine + ATP
-
 Drosophila melanogaster thymidine 5'-phosphate + ADP
-
 ?
2.7.1.145 thymidine + ATP
-
 Drosophila melanogaster S-2 thymidine 5'-phosphate + ADP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.145 monomer 1 * 29000, SDS-PAGE Drosophila melanogaster

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.145 additional information
-
 additional information
-
 Drosophila melanogaster

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.145 0.001
-
 thymidine pH 8.0, 22°C, competitive with 2'-deoxycytidine Drosophila melanogaster
2.7.1.145 0.001
-
 2'-deoxycytidine pH 8.0, 22°C, competitive with thymidine Drosophila melanogaster
2.7.1.145 0.005
-
 TTP pH 8.0, 22°C Drosophila melanogaster