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Literature summary extracted from

  • Arrowsmith, D.A.; de Silva, J.
    Characterisation of two tomato fruit-expressed cDNAs encoding xyloglucan endo-transglycosylase (1995), Plant Mol. Biol., 28, 391-403.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.207 small multi-gene family encodes XET: tXET–B1, -B2, -B3 and -B4, tXET-B2 is cloned, sequenced and overexpressed in Escherichia coli, nucleotide and amino acid sequence of tXET-B1 Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.207 30460
-
 x * 30460, tXET-B2 Solanum lycopersicum
2.4.1.207 30493
-
 x * 30493, tXET-B1 Solanum lycopersicum
2.4.1.207 32000
-
 x * 32000, His-tagged recombinant tXET-B2 with predicted molecular mass of 32 kDa Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.207 donor xyloglucan + xyloglucan acceptor Solanum lycopersicum important component of cell wall metabolism, particularly expanding tissue and ripening fruits, study on the role in tomato fruit ripening and vegetative growth, tXET-B1 may incorporate new xyloglucan into the existing network ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.207 Solanum lycopersicum
-
 cv. Murrieta
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.207 glycoprotein tXET-B1 and tXET-B2 with N-glycosylation sites Solanum lycopersicum
2.4.1.207 proteolytic modification tXET-B1 and tXET-B2 contain a hydrophobic signal peptide of 20 and 18 amino acids, respectively, typical of an exported protein Solanum lycopersicum

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.207 His-tagged recombinant tXET-B2, expressed in Escherichia coli Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.207 fruit tXET-B1 is most abundant in pink fruit pericarp Solanum lycopersicum
-
2.4.1.207 stem tXET-B1 is detected in stems Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.207 donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Solanum lycopersicum ?
-
 ?
2.4.1.207 donor xyloglucan + xyloglucan acceptor important component of cell wall metabolism, particularly expanding tissue and ripening fruits, study on the role in tomato fruit ripening and vegetative growth, tXET-B1 may incorporate new xyloglucan into the existing network Solanum lycopersicum ?
-
 ?
2.4.1.207 additional information tXET-B2 has no hydrolytic activity Solanum lycopersicum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.207 ? x * 30493, tXET-B1 Solanum lycopersicum
2.4.1.207 ? x * 32000, His-tagged recombinant tXET-B2 with predicted molecular mass of 32 kDa Solanum lycopersicum
2.4.1.207 ? x * 30460, tXET-B2 Solanum lycopersicum