Literature summary extracted from
Schröder, R.; Atkinson, R.G.; Langenkämper, G.; Redgwell, R.J.
Biochemical and molecular characterisation of xyloglucan endotransglycosylase from ripe kiwifruit (1998), Planta, 204, 242-251.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.4.1.207 |
6 isoforms, AdXET1-6 gene family is cloned, a full-length cDNA, AdXET5, is cloned, sequenced and overexpressed as active XET in Escherichia coli, purified XET is encoded by AdXET6 |
Actinidia deliciosa |
General Stability
EC Number |
General Stability |
Organism |
---|
2.4.1.207 |
loss of activity if frozen and stored at -80°C |
Actinidia deliciosa |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.4.1.207 |
Ag+ |
1 mM |
Actinidia deliciosa |
|
2.4.1.207 |
Hg2+ |
at least 10 mM |
Actinidia deliciosa |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
2.4.1.207 |
additional information |
- |
additional information |
Km: 0.6 mg/ml for kiwifruit xyloglucan |
Actinidia deliciosa |
|
2.4.1.207 |
0.1 |
- |
Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol |
reduced xyloglucan-derived heptasaccharide XXXG-ol, derived from kiwifruit xyloglucan |
Actinidia deliciosa |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
2.4.1.207 |
cell wall |
most of the XET activity is strongly associated with the cell wall |
Actinidia deliciosa |
5618 |
- |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.4.1.207 |
34000 |
- |
x * 34000, predicted MW of the mature protein encoded by AdXET5 is 31.8 kDa, SDS-PAGE |
Actinidia deliciosa |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.4.1.207 |
donor xyloglucan + xyloglucan acceptor |
Actinidia deliciosa |
XET and xyloglucan may play a role in the cell wall changes that accompany fruit softening during ripening, substrates for XET action are located in the cell wall |
? |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.4.1.207 |
Actinidia deliciosa |
- |
ripe kiwifruit, [A. Chev.] C.F. Liang et A.R. Ferguson var. deliciosa cv. Hayward, 6 isoenzymes: AdXET1-6 gene family |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
2.4.1.207 |
glycoprotein |
AdXET5 and AdXET6: asparagine-linked carbohydrate, N-glycosylated, glycosylation of the protein is not required for endo-transglycosylase activity |
Actinidia deliciosa |
2.4.1.207 |
proteolytic modification |
21 amino acids signal peptide at the N-terminus is cleaved to produce the mature protein |
Actinidia deliciosa |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.4.1.207 |
3000fold, AdXET6 |
Actinidia deliciosa |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
2.4.1.207 |
fruit |
core tissue of ripe kiwifruit |
Actinidia deliciosa |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
2.4.1.207 |
additional information |
- |
- |
Actinidia deliciosa |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
2.4.1.207 |
-80°C, partially purified XET, ConA-eluate stage, 4 weeks, stable |
Actinidia deliciosa |
2.4.1.207 |
-80°C, purified XET, loss of activity |
Actinidia deliciosa |
2.4.1.207 |
4°C, purified XET, 24 h, stable |
Actinidia deliciosa |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.4.1.207 |
donor xyloglucan + acceptor xyloglucan |
kiwifruit xyloglucan donor, acceptor: XXXG-ol, a reduced heptasaccharide derived from kiwifruit xyloglucan |
Actinidia deliciosa |
? |
- |
? |
|
2.4.1.207 |
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol |
XXXG-ol |
Actinidia deliciosa |
? |
- |
? |
|
2.4.1.207 |
donor xyloglucan + xyloglucan acceptor |
XET and xyloglucan may play a role in the cell wall changes that accompany fruit softening during ripening, substrates for XET action are located in the cell wall |
Actinidia deliciosa |
? |
- |
? |
|
2.4.1.207 |
additional information |
in absence of reduced xyloglucan-derived heptasaccharide XXXG-ol enzyme depolymerizes xyloglucan by hydrolysis and in the presence of it by both hydrolysis and endotransglycosylation |
Actinidia deliciosa |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.4.1.207 |
? |
x * 34000, predicted MW of the mature protein encoded by AdXET5 is 31.8 kDa, SDS-PAGE |
Actinidia deliciosa |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
2.4.1.207 |
22 |
- |
assay at |
Actinidia deliciosa |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.4.1.207 |
5.5 |
5.8 |
- |
Actinidia deliciosa |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
2.4.1.207 |
5 |
8 |
active between |
Actinidia deliciosa |