Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ogawa, T.; Fukuda, M.
    Occurrence of D-amino acid aminotransferase in pea seedlings (1973), Biochem. Biophys. Res. Commun., 52, 998-1002.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.21 D-cycloserine
-
 Pisum sativum
2.6.1.21 hydroxylamine 1 mM, 74% inhibition Pisum sativum
2.6.1.21 phenylhydrazine 1 mM; 30% inhibition Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.21 2.7
-
 D-alanine pH 8.3, 37°C Pisum sativum
2.6.1.21 4.9
-
 2-oxoglutarate pH 8.3, 37°C Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.21 65000
-
 gel filtration Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.21 Pisum sativum
-
 var Alaska
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.21 ammonium sulfate, DEAE-Sephadex, partial purification Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.21 seedling
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.21 0.088
-
-
 Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.21 D-2-aminobutanoate + pyruvate
-
 Pisum sativum 2-oxobutanoate + D-alanine
-
 ?
2.6.1.21 D-alanine + 2-oxoglutarate
-
 Pisum sativum pyruvate + D-glutamate
-
 r
2.6.1.21 D-aminobutanoate + 2-oxoglutarate
-
 Pisum sativum oxobutanoate + D-glutamate
-
 ?
2.6.1.21 D-aspartate + pyruvate
-
 Pisum sativum 2-oxosuccinate + D-alanine
-
 ?
2.6.1.21 D-glutamate + pyruvate
-
 Pisum sativum 2-oxoglutarate + D-alanine
-
 r
2.6.1.21 D-lysine + pyruvate weak activity Pisum sativum ? + D-alanine
-
 ?
2.6.1.21 D-methionine + 2-oxoglutarate weak activity Pisum sativum 4-methylsulfanyl-2-oxobutanoate + D-glutamate
-
 ?
2.6.1.21 D-methionine + pyruvate
-
 Pisum sativum 4-methylsulfanyl-2-oxobutanoate + L-alanine
-
 ?
2.6.1.21 D-phenylalanine + 2-oxoglutarate
-
 Pisum sativum phenylpyruvate + D-glutamate
-
 ?
2.6.1.21 D-phenylalanine + pyruvate weak activity Pisum sativum phenylpyruvate + D-alanine
-
 ?
2.6.1.21 D-tryptophan + 2-oxoglutarate
-
 Pisum sativum 3-indole-2-oxopropanoate + D-glutamate
-
 ?
2.6.1.21 D-tryptophan + pyruvate
-
 Pisum sativum 3-indole-2-oxopropanoate + D-alanine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.21 37
-
 assay at Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.21 8.2 8.8 with D-alanine and 2-oxoglutarate Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.21 pyridoxal 5'-phosphate addition of pyridoxal phosphate to the final preparation slightly stimulates Pisum sativum