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Literature summary extracted from

  • Rech, J.; Crouzet, J.
    Partial purification and initial studies of the tomato L-alanine:2-oxoglutarate aminotransferase (1974), Biochim. Biophys. Acta, 350, 392-399.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.2 during the purification procedure the enzyme needs protective substances, i.e. glycerol, 2-mercaptoethanol and alanine Solanum lycopersicum
2.6.1.2 uneffected by repeated freezing and thawing Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.2 0.09
-
 pyruvate pH 7.25, 37°C Solanum lycopersicum
2.6.1.2 0.28
-
 2-oxoglutarate pH 7.25, 37°C Solanum lycopersicum
2.6.1.2 2.3
-
 glutamate pH 7.25, 37°C Solanum lycopersicum
2.6.1.2 2.8
-
 alanine pH 7.25, 37°C Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.2 soluble
-
 Solanum lycopersicum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.2 100000
-
 gel filtration Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.2 Solanum lycopersicum
-
 tomato, 2 alanine aminotransferases, soluble and Triton X-100 extractable
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.2 ammonium sulfate, DEAE-Sephadex, Sephadex G-200, partial purification Solanum lycopersicum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.2 L-alanine + 2-oxoglutarate = pyruvate + L-glutamate ping pong bi bi mechanism Solanum lycopersicum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.2 fruit
-
 Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.2 120
-
-
 Solanum lycopersicum

Storage Stability

EC Number Storage Stability Organism
2.6.1.2 -20°C, stable for several months Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.2 L-alanine + 2-oxoglutarate rate of the forward reaction is 4 times higher than the rate of the reverse reaction, no activity with aspartate, glycine, threonine, leucine, methionine, arginine, phenylalanine, tyrosine, tryptophan, glyoxylate and 2-oxo-3-methylvalerate Solanum lycopersicum pyruvate + L-glutamate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.2 23
-
 assay at Solanum lycopersicum
2.6.1.2 37
-
 assay at Solanum lycopersicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.2 50
-
 quickly inactivated, 100 mM alanine gives slight protection Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.2 7.3 7.5
-
 Solanum lycopersicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.2 7 7.5 rapid decrease of activity below pH 7 and above pH 7.5 Solanum lycopersicum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.2 6.5
-
 strong loss of activity below Solanum lycopersicum