EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.1 | 0.00025 | - |
pyridoxyl 5'-phosphate | pH 7.6, 25°C | Escherichia coli | |
2.6.1.1 | 0.071 | - |
2-oxoglutarate | pH 7.6, 25°C | Escherichia coli | |
2.6.1.1 | 0.37 | - |
oxaloacetate | pH 7.6, 25°C | Escherichia coli | |
2.6.1.1 | 1.43 | - |
L-tyrosine | pH 7.6, 25°C | Escherichia coli | |
2.6.1.1 | 2.17 | - |
L-phenylalanine | - |
Escherichia coli | |
2.6.1.1 | 4.4 | - |
L-aspartate | - |
Escherichia coli | |
2.6.1.1 | 5 | - |
L-tryptophan | pH 7.6, 25°C | Escherichia coli | |
2.6.1.57 | 0.33 | - |
L-phenylalanine | - |
Escherichia coli | |
2.6.1.57 | 0.62 | - |
L-tyrosine | - |
Escherichia coli | |
2.6.1.57 | 2.5 | - |
2-oxoglutarate | - |
Escherichia coli | |
2.6.1.57 | 3.13 | - |
oxaloacetate | - |
Escherichia coli | |
2.6.1.57 | 10 | - |
L-tryptophan | - |
Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.1 | 82000 | - |
PAGE | Escherichia coli |
2.6.1.57 | 88000 | - |
- |
Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.1 | L-aspartate + 2-oxoglutarate | Escherichia coli | - |
oxaloacetate + L-glutamate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.1 | Escherichia coli | - |
- |
- |
2.6.1.57 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.1 | - |
Escherichia coli |
2.6.1.57 | - |
Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
2.6.1.1 | 4°C, stable for several weeks | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.1 | L-aspartate + 2-oxoglutarate | - |
Escherichia coli | oxaloacetate + L-glutamate | - |
? | |
2.6.1.1 | L-aspartate + 2-oxoglutarate | - |
Escherichia coli | oxaloacetate + L-glutamate | - |
r | |
2.6.1.1 | L-phenylalanine + 2-oxoglutarate | - |
Escherichia coli | 2-oxo-3-phenylpropionic acid + L-glutamate | i.e. phenylpyruvate | ? | |
2.6.1.1 | L-tryptophan + 2-oxoglutarate | - |
Escherichia coli | 3-indole-2-oxopropionic acid + L-glutamate | - |
? | |
2.6.1.1 | L-tyrosine + 2-oxoglutarate | - |
Escherichia coli | 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate | i.e. 4-hydroxyphenylpyruvate | ? | |
2.6.1.57 | L-phenylalanine + 2-oxoglutarate | - |
Escherichia coli | phenylpyruvate + L-glutamate | - |
? | |
2.6.1.57 | L-tryptophan + 2-oxoglutarate | - |
Escherichia coli | 3-indole-2-oxopropanoate + L-glutamate | - |
r | |
2.6.1.57 | L-tyrosine + 2-oxoglutarate | - |
Escherichia coli | p-hydroxyphenylpyruvate + L-glutamate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.6.1.1 | dimer | 2 * 42000-45000, SDS-PAGE | Escherichia coli |
2.6.1.57 | dimer | 2 * 42000-45000, SDS-PAGE | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.1 | More | EC 2.6.1.57 may be converted to EC 2.6.1.1 by controlled proteolysis with subtilisin, the 2 enzymes are encoded by 2 different genes with high sequence homology | Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.57 | 37 | - |
assay at | Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.1 | 55 | - |
10 min, 48% loss of activity with tyrosine, 36% loss of activity with phenylalanine | Escherichia coli |
2.6.1.57 | 55 | - |
10 min, less than 10% of original activity towards tyrosine and phenylalanine | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.1 | 8 | - |
formation of aspartate | Escherichia coli |
2.6.1.1 | 8.5 | - |
substrates L-phenylalanine or L-tryptophan | Escherichia coli |
2.6.1.1 | 9 | - |
substrate L-tyrosine | Escherichia coli |
2.6.1.57 | 7.5 | - |
tryptophan | Escherichia coli |
2.6.1.57 | 8 | - |
with tyrosine | Escherichia coli |
2.6.1.57 | 8 | - |
with phenylalanine | Escherichia coli |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.57 | 6 | 9.5 | approx. 45% of maximal activity at pH 6.0,: approx. 60% of maximal activity at pH 9.5, phenylalanine | Escherichia coli |
2.6.1.57 | 6 | 10 | approx. 40% of maximal activity at pH 6.0, tryptophan | Escherichia coli |
2.6.1.57 | 6 | 10 | approx. 25% of maximal activity at pH 10.0, tryptophan | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.1 | pyridoxal 5'-phosphate | a pyridoxal 5'-phosphate protein | Escherichia coli | |
2.6.1.57 | pyridoxal 5'-phosphate | Km: 0.01 mM | Escherichia coli | |
2.6.1.57 | pyridoxal 5'-phosphate | a pyridoxal phosphate protein | Escherichia coli |