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Literature summary extracted from
- Isolation and partial purification of prophenoloxidase from Daucus carota L. cell cultures (1985), Plant Physiol., 78, 730-733.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.18.1 | soluble | - |
Daucus carota | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.1 | Ca2+ | - |
Nicotiana tabacum |  |
| 1.14.18.1 | Ca2+ | - |
Xanthisma gracile | |
| 1.14.18.1 | Ca2+ | inactive enzyme is maximally activated by 10 mM Ca2+ | Daucus carota | |
| 1.14.18.1 | copper | - |
Daucus carota | |
| 1.14.18.1 | Mn2+ | activation above 1 mM Mn2+ | Daucus carota | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | Daucus carota | - |
- |
- |
| 1.14.18.1 | Nicotiana tabacum | - |
tobacco | - |
| 1.14.18.1 | Xanthisma gracile | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.18.1 | ammonium sulfate, DEAE-cellulose, partially purified | Nicotiana tabacum |
| 1.14.18.1 | ammonium sulfate, DEAE-cellulose, partially purified | Daucus carota |
| 1.14.18.1 | ammonium sulfate, DEAE-cellulose, partially purified | Xanthisma gracile |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | cell culture | - |
Daucus carota | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | additional information | - |
3314 units, 1 unit is the amount of enzyme causing an increase in absorbance at 490 nm of 0.001/min/ml | Daucus carota |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.1 | 3,4,5-trihydroxybenzoic acid + O2 | trivial name gallic acid | Daucus carota | ? | - |
? | |
| 1.14.18.1 | 4-methylcatechol + O2 | - |
Nicotiana tabacum | 4-methyl-o-benzoquinone + H2O | - |
? | |
| 1.14.18.1 | 4-methylcatechol + O2 | - |
Daucus carota | 4-methyl-o-benzoquinone + H2O | - |
? | |
| 1.14.18.1 | catechol + 1/2 O2 | - |
Daucus carota | 1,2-benzoquinone + H2O | - |
? | |
| 1.14.18.1 | chlorogenic acid + O2 | - |
Daucus carota | chlorogenoquinone + H2O | - |
? | |
| 1.14.18.1 | dopa + 1/2 O2 | - |
Daucus carota | dopaquinone + H2O | - |
? | |
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