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Literature summary extracted from

  • Yoon, M-Y.; Park, J.H.; Choi, K.J.; Kim, J.M.; Kim, Y.O.; Park, J.B.; Kyung, J.B.
    Purification and characterization of aspartase from Hafnia alvei (1998), J. Biochem. Mol. Biol., 31, 345-349.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.1 Ca2+ 10 mM, 18% loss of activity Hafnia alvei
4.3.1.1 Zn2+ 0.0005 mM, 40% loss of activity Hafnia alvei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.1 Mg2+ absolutely required Hafnia alvei
4.3.1.1 Mg2+ activates at the alkine pH Hafnia alvei
4.3.1.1 Mn2+ required at alkine pH Hafnia alvei
4.3.1.1 additional information
-
 Hafnia alvei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.1 55000
-
 4 * 55000, SDS-PAGE Hafnia alvei
4.3.1.1 173000
-
 sedimentation equilibrium analysis Pseudomonas fluorescens
4.3.1.1 198000
-
 pore gradient polyacrylamide Hafnia alvei

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.1 Hafnia alvei
-
 ATCC 9760
-
4.3.1.1 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.1
-
 Hafnia alvei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.1 236.8
-
-
 Hafnia alvei

Storage Stability

EC Number Storage Stability Organism
4.3.1.1 -20°C, stable for a few weeks Hafnia alvei
4.3.1.1 -70°C stable over 3 months Hafnia alvei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.1 L-aspartate r Pseudomonas fluorescens fumarate + NH3
-
 ?
4.3.1.1 L-aspartate r Hafnia alvei fumarate + NH3
-
 ?
4.3.1.1 L-aspartate natural substrate Pseudomonas fluorescens fumarate + NH3
-
 ?
4.3.1.1 L-aspartate natural substrate Hafnia alvei fumarate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.1 tetramer
-
 Pseudomonas fluorescens
4.3.1.1 tetramer 4 * 55000, SDS-PAGE Hafnia alvei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.1 45
-
 maximum activity Hafnia alvei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.1.1 15 60 15C: 90% loss of maximal activity, 60C: 90% loss of activity Hafnia alvei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.1 7
-
 in the absence of MgCl2 Hafnia alvei
4.3.1.1 8.5
-
 in the presence of 20 mM MgCl2 Hafnia alvei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.3.1.1 5.5 9
-
 Hafnia alvei