Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hurlbert, R.E.; Jakoby, W.B.
    Tartaric acid metabolism (1965), J. Biol. Chem., 240, 2772-2777.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.1.32 100 mM GSH plus 1mM Fe2+ optimal reactivation of inactive enzyme Pseudomonas putida
4.2.1.32 crude cell-extract completely loses activity in open tubes in ice bath for 2-4 h, activity restored after incubation at 25°C for 30 min, extent of activation decreases with each succeeding cycle of inactivation and reactivation, loss of activity after treatment with EDTA or after dialysis Pseudomonas putida
4.2.1.32 Cysteine 100 mM Cysteine plus 1 mM Fe2+ optimal reactivation of inactive enzyme Pseudomonas putida
4.2.1.32 equimolar mixture of 50 mM GSH and 50 mM Cysteine plus 1 mM Fe2+ optimum reactivation of inactive enzyme Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.32 (R,S)-tartrate competitive Pseudomonas putida
4.2.1.32 CdCl2
-
 Pseudomonas putida
4.2.1.32 CoCl2
-
 Pseudomonas putida
4.2.1.32 CuCl2
-
 Pseudomonas putida
4.2.1.32 cysteine 35% inhibition at 50 mM Pseudomonas putida
4.2.1.32 D-malic acid K-salt of Pseudomonas putida
4.2.1.32 glutathione 13% inhibition at 50 mM Pseudomonas putida
4.2.1.32 HgCl2
-
 Pseudomonas putida
4.2.1.32 L-Malic acid K-salt of Pseudomonas putida
4.2.1.32 L-threonic acid K-salt of Pseudomonas putida
4.2.1.32 Tartronic acid K-salt of Pseudomonas putida
4.2.1.32 Urea
-
 Pseudomonas putida
4.2.1.32 ZnCl2
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.32 additional information
-
 additional information reducing conditions: 2 KM-values/biphasic curve, presence of air: only one higher KM-value obtained Pseudomonas putida
4.2.1.32 0.81
-
 (R,R)-Tartrate relatively anaerobic conditions Pseudomonas putida
4.2.1.32 2.1
-
 (R,R)-Tartrate both, under anaerobic conditions and in the presence of air Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.32 Fe2+ addition of either GSH or cysteine plus Fe2+ reactivates inactive enzyme, optimum Fe2+ concentration 1 mM, Fe2+ only required for activation Pseudomonas putida
4.2.1.32 additional information requirement for Fe2+ cannot be replaced by Mg2+, Ba2+, Mn2+, Co2+, Ni2+, Zn2+, Cu2+, Al3+, BO32-, MoO42- at concentrations of 0.01 mM and 1 mM Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.32 39000
-
 sucrose density gradient centrifugation, inactive form. Aggregation of the four smaller units forms the active protein Pseudomonas putida
4.2.1.32 145000
-
 sucrose density gradient centrifugation, active form. Enzyme exists as an aggregate of smaller protein or proteins, the latter possessing no enzyme activity Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.32 (R,R)-Tartrate Pseudomonas putida
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.32 Pseudomonas putida
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.2.1.32 reactivation of the inactive enzyme is seriously inhibited by exposure to air Pseudomonas putida

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.32 partial Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.32 (R,R)-Tartrate
-
 Pseudomonas putida ?
-
 ?
4.2.1.32 L-(+)-Tartrate
-
 Pseudomonas putida Oxaloacetate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.32 monomer 1 * 39000 sucrose density gradient centrifugation, inactive form, activation by aggregation of 4 monomeres Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.32 25
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.32 8.5
-
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.32 7.6 9.4 50% of maximal activity at pH 7.6 and at pH 9.4 Pseudomonas putida