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Literature summary extracted from
- L-(+)-Tartaric acid dehydrase (1966), Methods Enzymol., 9, 680-682.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.2.1.32 | no significant loss of activity after repeated freezing and thawing | Pseudomonas putida |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.32 | (R,S)-tartrate | competitive | Pseudomonas putida |  |
| 4.2.1.32 | EDTA | - |
Pseudomonas putida | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.32 | additional information | - |
additional information | reducing conditions: 2 KM-values/biphasic curve, presence of air: only one higher KM-value obtained | Pseudomonas putida | |
| 4.2.1.32 | 0.8 | - |
(R,R)-Tartrate | in the presence of air | Pseudomonas putida | |
| 4.2.1.32 | 2 | - |
(R,R)-Tartrate | elimination of air | Pseudomonas putida | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.32 | Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.32 | partial | Pseudomonas putida |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.2.1.32 | -20°C, under reduced pressure and minimized contact with air several months | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.32 | (R,S)-Tartrate | no activity with D-(-)-tartrate, (R,S)-tartrate | Pseudomonas putida | Oxaloacetate + H2O | - |
? | |
| 4.2.1.32 | L-(+)-Tartrate | no activity with D-(-)-tartrate, (R,S)-tartrate | Pseudomonas putida | Oxaloacetate + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.32 | monomer | - |
Pseudomonas putida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.32 | 25 | - |
assay at | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.32 | 8.5 | - |
- |
Pseudomonas putida |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.32 | 7.6 | 9.4 | 50% of maximal activity at pH 7.6 and at pH 9.4 | Pseudomonas putida |
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Release 2023.1 (January 2023)
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