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Literature summary extracted from

  • White, W.L.B.; Ariaz-Garzon, D.I.; McMahon, J.M.; Sayre, R.T.
    Cyanogenesis in Cassava. The role of hydroxynitrile lyase in root cyanide production (1998), Plant Physiol., 116, 1219-1225.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.47 0.93
-
 CN-
-
 Manihot esculenta

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.2.47 cell wall
-
 Manihot esculenta 5618
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.47 28500
-
 2 * 28500, SDS-PAGE Manihot esculenta
4.1.2.47 50100
-
 gel filtration Manihot esculenta

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.47 Manihot esculenta P52705
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.47 leaf
-
 Manihot esculenta
-
4.1.2.47 root 6% of the activity of leaves Manihot esculenta
-
4.1.2.47 stem 2% of the activity of leaves Manihot esculenta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.47 119.7
-
-
 Manihot esculenta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.47 2-hydroxy-2-methylpropanenitrile acetone cyanohydrin spontaneously decomposes to acetone and cyanide at pH above 5.0 or temperatures above 35 C Manihot esculenta acetone + HCN
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.47 dimer 2 * 28500, SDS-PAGE Manihot esculenta