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Literature summary extracted from

  • Shiomi, N.; Izawa, M.
    Purification and characterization of sucrose:sucrose 1-fructosyltransferase from the roots of Asparagus (Asparagus officinalis L.) (1980), Agric. Biol. Chem., 44, 603-614.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.99 Ag+ 1 mM, 71% inhibition Asparagus officinalis
2.4.1.99 Hg2+ 0.1 mM, 81% inhibition Asparagus officinalis
2.4.1.99 Mn2+ 1.0 mM, 75% inhibition Asparagus officinalis
2.4.1.99 PCMB 0.12 mM, 22% inhibition Asparagus officinalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.99 0.11
-
 sucrose
-
 Asparagus officinalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.99 65000
-
 gel filtration Asparagus officinalis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.99 Asparagus officinalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.99
-
 Asparagus officinalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.99 root
-
 Asparagus officinalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.99 2.97
-
-
 Asparagus officinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.99 sucrose + 6G(1-beta-fructofuranosyl)2-sucrose
-
 Asparagus officinalis D-glucose + 1F-beta-fructofuranosyl-6G(1-beta-fructofuranosyl)2-sucrose
-
 ?
2.4.1.99 sucrose + neokestose
-
 Asparagus officinalis D-glucose + 1F,6G-di-beta-fructofuranosylsucrose
-
 ?
2.4.1.99 sucrose + sucrose
-
 Asparagus officinalis D-glucose + 1F-beta-D-fructosylsucrose
-
 ?
2.4.1.99 sucrose + sucrose
-
 Asparagus officinalis D-glucose + 1F-beta-D-fructosylsucrose
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.99 30
-
 pH 4.0-8.0, 30 min, stable Asparagus officinalis
2.4.1.99 45
-
 pH 5.0-6.5, 50% loss of activity after 30 min, pH 4.0 and pH 8.0, more than 90% loss of activity after 30 min Asparagus officinalis
2.4.1.99 60
-
 10 min, 82% loss of activity Asparagus officinalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.99 5
-
-
 Asparagus officinalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.99 4 8 30°C, stable Asparagus officinalis