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Literature summary extracted from

  • Van Hijum, S.A.F.T.M.; van Geel-Schutten, G.H.; Rahaoui, H.; van der Maarel, M.J.E.C.; Dijkhuizen, L.
    Characterization of a novel fructosyltransferase from Lactobacillus reuteri that synthesizes high molecular weight inulin and inulin oligosaccharides (2002), Appl. Environ. Microbiol., 68, 4390-4398.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.9 expression of the full-length protein and a truncated protein with a 100 amino acid truncation at the C-terminus from amino acid 699 onwards, with a C-terminal His-tag, InuDELTA699His Limosilactobacillus reuteri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup Limosilactobacillus reuteri
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 alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.9 Limosilactobacillus reuteri
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-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.9 recombinant enzyme Limosilactobacillus reuteri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.9 103.7
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-
 Limosilactobacillus reuteri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup
-
 Limosilactobacillus reuteri alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
 ?
2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup the recombinant truncated enzyme produces both the fructooligosaccharides 1-kestose and nystose and high-molecular-weight-fructan with beta-(2, 1) linkages. The wild-type enzyme only produces 1-kestose, 95%, and nystose, 5% Limosilactobacillus reuteri alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
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 ?