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Literature summary extracted from

  • Arai, M.; Tanaka, K.; Kawaguchi, T.
    Purification and properties of cellodextrin phosphorylase from Clostridium thermocellum (1994), J. Ferment. Bioeng., 77, 239-242.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.4.1.49 synthesis enzyme is a good tool for the synthesis of cellodextrins Acetivibrio thermocellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.49 Ag+ slight inhibition at 1 mM Acetivibrio thermocellus
2.4.1.49 Cd2+ slight inhibition at 1 mM Acetivibrio thermocellus
2.4.1.49 Cu2+ slight inhibition at 1 mM Acetivibrio thermocellus
2.4.1.49 Hg2+ slight inhibition at 1 mM Acetivibrio thermocellus
2.4.1.49 N-ethylmaleimide 26% remaining activity at 10 mM Acetivibrio thermocellus
2.4.1.49 Zn2+ slight inhibition at 1 mM Acetivibrio thermocellus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.49 dithiothreitol 143fold activation at 10 mM Acetivibrio thermocellus
2.4.1.49 Sr2+ slight activation at 1 mM Acetivibrio thermocellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.49 additional information
-
 amino acid composition Acetivibrio thermocellus
2.4.1.49 additional information
-
 N-terminal amino acid sequence Acetivibrio thermocellus
2.4.1.49 105000
-
 SDS-PAGE Acetivibrio thermocellus
2.4.1.49 200000
-
 gel filtration Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.49 (1,4-beta-D-glucosyl)n + phosphate Acetivibrio thermocellus
-
 (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.49 Acetivibrio thermocellus
-
 strain ATCC 27405
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.49
-
 Acetivibrio thermocellus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.49 64.2
-
 purified enzyme Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.49 (1,4-beta-D-glucosyl)n + phosphate
-
 Acetivibrio thermocellus (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.49 phosphate + cellopentaose
-
 Acetivibrio thermocellus cellotetraose + glucose 1-phosphate
-
 r
2.4.1.49 phosphate + cellotetraose
-
 Acetivibrio thermocellus cellotriose + glucose 1-phosphate
-
 r
2.4.1.49 phosphate + cellotriose
-
 Acetivibrio thermocellus cellobiose + glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.49 monomer 1 * 105000, SDS-PAGE Acetivibrio thermocellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.49 60
-
-
 Acetivibrio thermocellus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.49 60
-
 pH 7.5, 10 min, stable Acetivibrio thermocellus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.49 6 9 37°C, 1 h, stable Acetivibrio thermocellus

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.1.49 additional information contains no pyridoxyl 5'-phosphate Acetivibrio thermocellus