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Literature summary extracted from

  • Cabib, E.; Kang, M.S.
    Fungal 1,3-beta-glucan synthase (1987), Methods Enzymol., 138, 637-642.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.34 bovine serum albumin enhances activity Saccharomyces cerevisiae
2.4.1.34 bovine serum albumin enhances activity Neurospora crassa
2.4.1.34 bovine serum albumin enhances activity Papiliotrema laurentii
2.4.1.34 bovine serum albumin enhances activity Wickerhamomyces anomalus
2.4.1.34 bovine serum albumin enhances activity Schizophyllum commune
2.4.1.34 bovine serum albumin enhances activity Exophiala dermatitidis
2.4.1.34 bovine serum albumin enhances activity Achlya ambisexualis
2.4.1.34 glycerol stimulate Saccharomyces cerevisiae
2.4.1.34 GTP
-
 Saccharomyces cerevisiae
2.4.1.34 GTP
-
 Neurospora crassa
2.4.1.34 GTP
-
 Papiliotrema laurentii
2.4.1.34 GTP
-
 Wickerhamomyces anomalus
2.4.1.34 GTP
-
 Schizophyllum commune
2.4.1.34 GTP
-
 Exophiala dermatitidis
2.4.1.34 GTP
-
 Achlya ambisexualis

General Stability

EC Number General Stability Organism
2.4.1.34 guanosine nucleotides prevent inactivation at 30°C Saccharomyces cerevisiae
2.4.1.34 guanosine nucleotides prevent inactivation at 30°C Neurospora crassa
2.4.1.34 guanosine nucleotides prevent inactivation at 30°C Wickerhamomyces anomalus
2.4.1.34 inactivation at 30°C is greatly accelerated by the presence of 1-2 mM EDTA Saccharomyces cerevisiae
2.4.1.34 inactivation at 30°C is greatly accelerated by the presence of 1-2 mM EDTA Neurospora crassa
2.4.1.34 inactivation at 30°C is greatly accelerated by the presence of 1-2 mM EDTA Wickerhamomyces anomalus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.34 N-ethylmaleimide 0.16 mM, partial inhibition Exophiala dermatitidis
2.4.1.34 N-ethylmaleimide 0.16 mM, partial inhibition Neurospora crassa
2.4.1.34 N-ethylmaleimide 0.16 mM, partial inhibition Papiliotrema laurentii
2.4.1.34 N-ethylmaleimide 0.16 mM, partial inhibition Saccharomyces cerevisiae
2.4.1.34 N-ethylmaleimide 0.16 mM, partial inhibition Schizophyllum commune
2.4.1.34 N-ethylmaleimide 0.16 mM, partial inhibition Wickerhamomyces anomalus
2.4.1.34 Showdomycin 0.1 mM, partial inhibition Exophiala dermatitidis
2.4.1.34 Showdomycin 0.1 mM, partial inhibition Neurospora crassa
2.4.1.34 Showdomycin 0.1 mM, partial inhibition Papiliotrema laurentii
2.4.1.34 Showdomycin 0.1 mM, partial inhibition Saccharomyces cerevisiae
2.4.1.34 Showdomycin 0.1 mM, partial inhibition Schizophyllum commune
2.4.1.34 Showdomycin 0.1 mM, partial inhibition Wickerhamomyces anomalus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.34 0.67
-
 UDPglucose
-
 Wickerhamomyces anomalus
2.4.1.34 0.8
-
 UDPglucose
-
 Schizophyllum commune
2.4.1.34 0.86
-
 UDPglucose
-
 Papiliotrema laurentii
2.4.1.34 1.8
-
 UDPglucose
-
 Exophiala dermatitidis
2.4.1.34 2.9
-
 UDPglucose
-
 Neurospora crassa
2.4.1.34 3.8
-
 UDPglucose
-
 Saccharomyces cerevisiae
2.4.1.34 7.1
-
 UDPglucose
-
 Achlya ambisexualis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n Saccharomyces cerevisiae
-
 UDP + (1,3-beta-D-glucosyl)n+1
-
 ?
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n Neurospora crassa
-
 UDP + (1,3-beta-D-glucosyl)n+1
-
 ?
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n Papiliotrema laurentii
-
 UDP + (1,3-beta-D-glucosyl)n+1
-
 ?
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n Wickerhamomyces anomalus
-
 UDP + (1,3-beta-D-glucosyl)n+1
-
 ?
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n Schizophyllum commune
-
 UDP + (1,3-beta-D-glucosyl)n+1
-
 ?
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n Exophiala dermatitidis
-
 UDP + (1,3-beta-D-glucosyl)n+1
-
 ?
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n Achlya ambisexualis
-
 UDP + (1,3-beta-D-glucosyl)n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.34 Achlya ambisexualis
-
-
-
2.4.1.34 Exophiala dermatitidis
-
-
-
2.4.1.34 Neurospora crassa
-
-
-
2.4.1.34 Papiliotrema laurentii
-
-
-
2.4.1.34 Saccharomyces cerevisiae
-
-
-
2.4.1.34 Schizophyllum commune
-
-
-
2.4.1.34 Wickerhamomyces anomalus
-
-
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.34 -80°C, several months Saccharomyces cerevisiae
2.4.1.34 -80°C, several months Neurospora crassa
2.4.1.34 -80°C, several months Papiliotrema laurentii
2.4.1.34 -80°C, several months Wickerhamomyces anomalus
2.4.1.34 -80°C, several months Schizophyllum commune
2.4.1.34 -80°C, several months Exophiala dermatitidis
2.4.1.34 -80°C, several months Achlya ambisexualis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n
-
 Saccharomyces cerevisiae UDP + (1,3-beta-D-glucosyl)n+1
-
 ?
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n
-
 Neurospora crassa UDP + (1,3-beta-D-glucosyl)n+1
-
 ?
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n
-
 Papiliotrema laurentii UDP + (1,3-beta-D-glucosyl)n+1
-
 ?
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n
-
 Wickerhamomyces anomalus UDP + (1,3-beta-D-glucosyl)n+1
-
 ?
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n
-
 Schizophyllum commune UDP + (1,3-beta-D-glucosyl)n+1
-
 ?
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n
-
 Exophiala dermatitidis UDP + (1,3-beta-D-glucosyl)n+1
-
 ?
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n
-
 Achlya ambisexualis UDP + (1,3-beta-D-glucosyl)n+1
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.34 7.5 8
-
 Saccharomyces cerevisiae