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Literature summary extracted from

  • Schmidt, J.; John, M.
    Starch metabolism in Pseudomonas stutzeri. II. Purification and properties of a dextrin glycosyl-transferase (D-enzyme) and amylomaltase (1979), Biochim. Biophys. Acta, 566, 100-114.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.25 2-mercaptoethanol D-enzyme, slight inhibition Stutzerimonas stutzeri
2.4.1.25 cyclomaltohexaose inhibition 25% Stutzerimonas stutzeri
2.4.1.25 dithiothreitol D-enzyme, slight inhibition Stutzerimonas stutzeri
2.4.1.25 EDTA D-enzyme, slight inhibition Stutzerimonas stutzeri
2.4.1.25 Fe2+ D-enzyme, 50% inhibition Stutzerimonas stutzeri
2.4.1.25 Hg2+ D-enzyme Stutzerimonas stutzeri
2.4.1.25 methyl-alpha-D-glucoside competitive inhibition Escherichia coli
2.4.1.25 p-chloromercuribenzoic acid D-enzyme, 30% inhibition Stutzerimonas stutzeri
2.4.1.25 phenyl-beta-D-glucoside competitive inhibition Escherichia coli
2.4.1.25 Tris D-enzyme, 50 mM, 35% inhibition Stutzerimonas stutzeri
2.4.1.25 Zn2+ D-enzyme Stutzerimonas stutzeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.25 7.1
-
 maltotetraose
-
 Stutzerimonas stutzeri
2.4.1.25 8.3
-
 maltose D-enzyme Stutzerimonas stutzeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.25 38000
-
 1 * 38000 + 1 * 47000, amylomaltase Stutzerimonas stutzeri
2.4.1.25 47000
-
 1 * 38000 + 1 * 47000, amylomaltase Stutzerimonas stutzeri
2.4.1.25 52000
-
 1 * 52000 + 1 * 63000, D-enzyme Stutzerimonas stutzeri
2.4.1.25 63000
-
 1 * 52000 + 1 * 63000, D-enzyme Stutzerimonas stutzeri
2.4.1.25 71000
-
-
 Escherichia coli
2.4.1.25 74000
-
 amylomaltase, gel filtration Stutzerimonas stutzeri
2.4.1.25 115000
-
 D-enzyme, gel filtration Stutzerimonas stutzeri

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Bacillus subtilis
-
-
-
2.4.1.25 Escherichia coli
-
-
-
2.4.1.25 Escherichia coli ML 30
-
-
-
2.4.1.25 Solanum tuberosum
-
 potato
-
2.4.1.25 Streptococcus sp.
-
-
-
2.4.1.25 Stutzerimonas stutzeri
-
 NRRL B3389
-
2.4.1.25 Stutzerimonas stutzeri NRRL B3389
-
 NRRL B3389
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25 both amylomaltase and D-enzyme Stutzerimonas stutzeri
2.4.1.25 E. coli ML 30, partially Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.4.1.25 4°C, stored as a suspension in 3.2 M ammonium sulfate, no significant loss of activity for at least 1 month Stutzerimonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 maltoheptaose + maltotriose D-enzyme Stutzerimonas stutzeri maltononaose + D-glucose
-
 ?
2.4.1.25 maltohexaose + maltodextrin only amylomaltase, no polymers larger than the initial maltodextrin substrate, maltohexaose is a good donor substrate, but unable to function as an acceptor Stutzerimonas stutzeri D-glucose + maltopentaose
-
 ?
2.4.1.25 maltohexaose + maltodextrin only amylomaltase, no polymers larger than the initial maltodextrin substrate, maltohexaose is a good donor substrate, but unable to function as an acceptor Stutzerimonas stutzeri NRRL B3389 D-glucose + maltopentaose
-
 ?
2.4.1.25 maltononaose + maltotriose D-enzyme Stutzerimonas stutzeri maltoundecaose + D-glucose
-
 ?
2.4.1.25 maltopentaose + maltodextrin
-
 Stutzerimonas stutzeri maltose + higher dextrins
-
 ?
2.4.1.25 maltopentaose + maltodextrin
-
 Stutzerimonas stutzeri NRRL B3389 maltose + higher dextrins
-
 ?
2.4.1.25 maltopentaose + maltotriose D-enzyme Stutzerimonas stutzeri maltoheptaose + D-glucose
-
 ?
2.4.1.25 maltopentaose + maltotriose amylomaltose Stutzerimonas stutzeri homologous 1,4-alpha-D-glucans
-
 ?
2.4.1.25 maltose + maltose
-
 Escherichia coli maltotriose + glucose
-
 ?
2.4.1.25 maltose + maltose D-enzyme Stutzerimonas stutzeri maltotriose + glucose
-
 ?
2.4.1.25 maltose + maltose
-
 Escherichia coli ML 30 maltotriose + glucose
-
 ?
2.4.1.25 maltotetraose + maltodextrin
-
 Stutzerimonas stutzeri maltose + higher dextrins
-
 ?
2.4.1.25 maltotetraose + maltodextrin
-
 Stutzerimonas stutzeri NRRL B3389 maltose + higher dextrins
-
 ?
2.4.1.25 maltotetraose + maltotriose D-enzyme Stutzerimonas stutzeri glucose + maltoheptaose + maltodecaose
-
 ?
2.4.1.25 maltotetraose + maltotriose amylomaltose Stutzerimonas stutzeri homologous 1,4-alpha-glucans
-
 ?
2.4.1.25 maltotetraose + maltotriose
-
 Solanum tuberosum D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 maltotetraose + maltotriose amylomaltose, most active with maltotetraose Stutzerimonas stutzeri D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 maltotriose + maltodextrin
-
 Stutzerimonas stutzeri maltose + higher dextrins
-
 ?
2.4.1.25 maltotriose + maltodextrin
-
 Stutzerimonas stutzeri NRRL B3389 maltose + higher dextrins
-
 ?
2.4.1.25 maltotriose + maltodextrin amylomaltase Stutzerimonas stutzeri maltopentaose + ?
-
 ?
2.4.1.25 maltotriose + maltodextrin amylomaltase Stutzerimonas stutzeri NRRL B3389 maltopentaose + ?
-
 ?
2.4.1.25 maltotriose + maltotriose D-enzyme Stutzerimonas stutzeri maltopentaose + D-glucose
-
 ?
2.4.1.25 maltotriose + maltotriose D-enzyme Stutzerimonas stutzeri D-glucose + maltopentaose + maltoheptaose + maltononaose + maltoundecaose
-
 ?
2.4.1.25 maltotriose + maltotriose amylomaltose Stutzerimonas stutzeri homologous alpha-1,4-D-glucans
-
 ?
2.4.1.25 additional information
-
 Escherichia coli ?
-
 ?
2.4.1.25 additional information maltose is not a donor substrate, only weak acceptor activity Solanum tuberosum ?
-
 ?
2.4.1.25 additional information D-enzyme, maltohexaose is no initial substrate Stutzerimonas stutzeri ?
-
 ?
2.4.1.25 additional information 2 glycosyltransferases, amylomaltase and D-enzyme Stutzerimonas stutzeri ?
-
 ?
2.4.1.25 additional information
-
 Escherichia coli ML 30 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.25 dimer 1 * 38000 + 1 * 47000, amylomaltase Stutzerimonas stutzeri
2.4.1.25 dimer 1 * 52000 + 1 * 63000, D-enzyme Stutzerimonas stutzeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.25 30
-
 D-enzyme Stutzerimonas stutzeri
2.4.1.25 37
-
 amylomaltase Stutzerimonas stutzeri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.25 37 42 D-enzyme, activity decreases rapidly above 37°C and is almost completely lost at 42°C Stutzerimonas stutzeri
2.4.1.25 50
-
 amylomaltase, rapidly inactivated at temperatures above Stutzerimonas stutzeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.25 6.9
-
-
 Escherichia coli
2.4.1.25 7.6 7.7 both amylomaltase and D-enzyme Stutzerimonas stutzeri