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Literature summary extracted from

  • Doukyu, N.; Kuwahara, H.; Aono, R.
    Isolation of Paenibacillus illinoisensis that produces cyclodextrin glucanotransferase resistant to organic solvents (2003), Biosci. Biotechnol. Biochem., 67, 334-340.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.19 Benzene relative activity 106% Paenibacillus illinoisensis
2.4.1.19 Cyclohexane
-
 Niallia circulans
2.4.1.19 Cyclohexane
-
 Paenibacillus macerans
2.4.1.19 Cyclohexane relative activity 102% Paenibacillus illinoisensis
2.4.1.19 decane increases the yield of cyclodextrin Paenibacillus illinoisensis
2.4.1.19 ethanol
-
 Niallia circulans
2.4.1.19 ethanol
-
 Paenibacillus macerans
2.4.1.19 ethanol
-
 Salimicrobium halophilum
2.4.1.19 ethanol increases yield of cyclodextrins, relative activity 104% Paenibacillus illinoisensis
2.4.1.19 ethanol BE101 produces twice as much cyclodextrin Bacillus sp. (in: Bacteria)
2.4.1.19 tert-butanol strain 251, increases the yield of cyclodextrin Niallia circulans
2.4.1.19 Toluene
-
 Niallia circulans
2.4.1.19 Toluene
-
 Paenibacillus macerans
2.4.1.19 Toluene
-
 Salimicrobium halophilum
2.4.1.19 Toluene relative residual activity 102%, relative activity 104% Paenibacillus illinoisensis

Application

EC Number Application Comment Organism
2.4.1.19 agriculture cyclodextrin production, agriculture chemistry Paenibacillus illinoisensis
2.4.1.19 nutrition important enzyme in food industry Bacillus sp. (in: Bacteria)
2.4.1.19 nutrition important enzyme in food industry Paenibacillus macerans
2.4.1.19 nutrition important enzyme in food industry Paenibacillus illinoisensis
2.4.1.19 pharmacology important enzyme in pharmaceutical industry Bacillus sp. (in: Bacteria)
2.4.1.19 pharmacology important enzyme in pharmaceutical industry Paenibacillus macerans
2.4.1.19 pharmacology important enzyme in pharmaceutical industry Paenibacillus illinoisensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.19 2-propanol
-
 Paenibacillus macerans
2.4.1.19 chloroform
-
 Paenibacillus macerans
2.4.1.19 ethanol BE 101 Bacillus sp. (in: Bacteria)
2.4.1.19 ethanol
-
 Paenibacillus macerans
2.4.1.19 methanol
-
 Paenibacillus macerans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.19 70000
-
-
 Salimicrobium halophilum
2.4.1.19 70000
-
 SDS-PAGE Paenibacillus illinoisensis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.4.1.19 2-propanol stable in presence, relative residual activity 82% Paenibacillus illinoisensis
2.4.1.19 2-propanol stable in presence, but not active, relative residual activity 96% Paenibacillus macerans
2.4.1.19 benzene relative residual activity 88% Paenibacillus illinoisensis
2.4.1.19 benzene relative residual activity 92% Paenibacillus macerans
2.4.1.19 chloroform stable in presence Paenibacillus illinoisensis
2.4.1.19 chloroform stable in presence, but not active Paenibacillus macerans
2.4.1.19 cyclohexane relative residual activity 88% Paenibacillus illinoisensis
2.4.1.19 cyclohexane relative residual activity 91% Paenibacillus macerans
2.4.1.19 decane decane, nonane, stable in presence Paenibacillus illinoisensis
2.4.1.19 diphenyl ether diphenyl ether, 90% activity in presence Paenibacillus illinoisensis
2.4.1.19 dodecane stable in presence Paenibacillus illinoisensis
2.4.1.19 Ethanol stable in presence, relative residual activity 84% Paenibacillus illinoisensis
2.4.1.19 Ethanol stable in presence, but not active, relative residual activity 99% Paenibacillus macerans
2.4.1.19 hexane n-hexane, relative residual activity 90% Paenibacillus macerans
2.4.1.19 hexane n-hexane, relative residual activity 84% Paenibacillus illinoisensis
2.4.1.19 Methanol stable in presence, but not active, relative residual activity 98% Paenibacillus macerans
2.4.1.19 Methanol stable in presence, relative residual activity 102% Paenibacillus illinoisensis
2.4.1.19 nonane decane, nonane, stable in presence Paenibacillus illinoisensis
2.4.1.19 octane n-octane, 90% activity in presence Paenibacillus illinoisensis
2.4.1.19 p-xylene p-xylene, relative residual activity 100% Paenibacillus macerans
2.4.1.19 toluene relative residual activity 100% Paenibacillus macerans
2.4.1.19 toluene relative residual activity 102% Paenibacillus illinoisensis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Bacillus ohbensis
-
-
-
2.4.1.19 Bacillus sp. (in: Bacteria)
-
 BE101
-
2.4.1.19 Bacillus sp. (in: Bacteria) BE101
-
 BE101
-
2.4.1.19 Brevibacterium sp.
-
 no. 9605
-
2.4.1.19 Brevibacterium sp. 9605
-
 no. 9605
-
2.4.1.19 Niallia circulans
-
-
-
2.4.1.19 Niallia circulans 251
-
-
-
2.4.1.19 Paenibacillus illinoisensis
-
 ST-12K
-
2.4.1.19 Paenibacillus illinoisensis ST-12K
-
 ST-12K
-
2.4.1.19 Paenibacillus macerans
-
-
-
2.4.1.19 Priestia megaterium
-
-
-
2.4.1.19 Salimicrobium halophilum
-
-
-
2.4.1.19 Thermoanaerobacterium thermosulfurigenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19
-
 Paenibacillus illinoisensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.19 1200
-
-
 Paenibacillus illinoisensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 soluble starch + glycosyl acceptor
-
 Priestia megaterium alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus macerans alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Thermoanaerobacterium thermosulfurigenes alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus ohbensis alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Niallia circulans alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin strain 251, product proportions 9: 82: 9 with addition of tert-butanol, 15: 65: 20 without solvent r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus illinoisensis alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin ratio 12: 82: 6 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus illinoisensis ST-12K alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin ratio 12: 82: 6 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) BE101 alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Niallia circulans 251 alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin strain 251, product proportions 9: 82: 9 with addition of tert-butanol, 15: 65: 20 without solvent r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Priestia megaterium beta-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) beta-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Niallia circulans beta-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus macerans beta-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 Thermoanaerobacterium thermosulfurigenes beta-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Salimicrobium halophilum beta-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus ohbensis beta-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus illinoisensis beta-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus illinoisensis ST-12K beta-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) BE101 beta-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Niallia circulans 251 beta-cyclodextrin
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 65
-
-
 Paenibacillus illinoisensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.19 20 80
-
 Paenibacillus illinoisensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.19 4 80 enzyme is stable from 4-45°C, still has 85% activity after 30 min at 60°C, 40% activity after 30 min at 70°C, loses almost all activity after 30 min at 80°C Paenibacillus illinoisensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 5 8
-
 Paenibacillus illinoisensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.19 5 9
-
 Paenibacillus illinoisensis
2.4.1.19 5 8
-
 Bacillus sp. (in: Bacteria)
2.4.1.19 5 8
-
 Paenibacillus macerans
2.4.1.19 5 8
-
 Paenibacillus illinoisensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.19 6 10
-
 Niallia circulans
2.4.1.19 6 10
-
 Paenibacillus illinoisensis