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Literature summary extracted from

  • Sburlati, A.; Cabib, E.
    Chitin synthetase 2, a presumptive participant in septum formation in Saccharomyces cerevisiae (1986), J. Biol. Chem., 261, 15147-15152.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.16 GlcNAc stimulates both Chs1 and Chs2 about 3fold Saccharomyces cerevisiae
2.4.1.16 papain fairly good activator of Chs1, poor activator of Chs2 Saccharomyces cerevisiae
2.4.1.16 Pronase stimulates both Chs1 and Chs2 Saccharomyces cerevisiae
2.4.1.16 proteinase B from Saccharomyces cerevisiae, stimulates Chs1, no effect on Chs2 Saccharomyces cerevisiae
2.4.1.16 proteinase K stimulates both Chs1 and Chs2 Saccharomyces cerevisiae
2.4.1.16 Staphylococcus V8 protease best activator of Chs2 in presence of Co2+, elicits little Mg2+-stimulatable activity Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.16 Co2+ inhibits Chs1 Saccharomyces cerevisiae
2.4.1.16 EDTA 0.1 mM, Chs1 and Chs2 are almost completely inhibited Saccharomyces cerevisiae
2.4.1.16 NaCl 0.5 M, 86% inhibition of Chs1 and 29% inhibition of Chs2 Saccharomyces cerevisiae
2.4.1.16 Polyoxin D Chs2 shows less sensitivity to inhibition than Chs1 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.16 0.8
-
 UDP-GlcNAc Chs1 Saccharomyces cerevisiae
2.4.1.16 0.9
-
 UDP-GlcNAc Chs1 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.16 plasma membrane most of the enzyme is associated with Saccharomyces cerevisiae 5886
-
2.4.1.16 protoplast
-
 Saccharomyces cerevisiae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.16 Co2+ in stimulation of Chs2 Co2+ is twice as effective on an enzyme activated by trypsin Saccharomyces cerevisiae
2.4.1.16 Mg2+ best stimulator of Chs1. Mg2+ and Mn2+ lead to similar maximal stimulation of Chs2 Saccharomyces cerevisiae
2.4.1.16 Mn2+ Mg2+ and Mn2+ lead to similar maximal stimulation of Chs2 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.16 UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n Saccharomyces cerevisiae chitin synthetase 2 is the physiological agent for chitin deposition in strains with a disrupted CHS1 gene UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.16 Saccharomyces cerevisiae
-
 chitin synthetase 2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.16 proteolytic modification pronase and proteinase K stimulates both Chs1 and Chs2. Proteinase B from Saccharomyces cerevisiae stimulates Chs1 and has no effect on Chs2, Staphylococcus V8 protease is the best activator of Chs2 in presence of Co2+, elicits little Mg2+-stimulatable activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.16 UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n
-
 Saccharomyces cerevisiae UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1 chitin ?
2.4.1.16 UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n chitin synthetase 2 is the physiological agent for chitin deposition in strains with a disrupted CHS1 gene Saccharomyces cerevisiae UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.16 0.003
-
 Polyoxin D Chs1 Saccharomyces cerevisiae
2.4.1.16 0.015
-
 Polyoxin D Chs2 Saccharomyces cerevisiae