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Literature summary extracted from

  • Breithaupt, T.B.; Light, R.J.
    Affinity chromatography and further characterization of the glucosyltransferases involved in hydroxydocosanoic acid sophoroside production in Candida bogoriensis (1982), J. Biol. Chem., 257, 9622-9628.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.158 glycerol, 20%, stabilizes Pseudohyphozyma bogoriensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.158 NaCl 0.2 mM: 50% inhibition of glucosyltransferase I, 0.25 mM: 50% inhibition of glucosyltransferase II Pseudohyphozyma bogoriensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.158 0.04
-
 UDPglucose glucosyltransferase I and II Pseudohyphozyma bogoriensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.158 50000
-
 gel filtration Pseudohyphozyma bogoriensis
2.4.1.158 52000
-
 1 * 52000, SDS-PAGE Pseudohyphozyma bogoriensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.158 UDPglucose + 13-(beta-D-glucopyranosyl)oxydocosanoic acid methyl ester Pseudohyphozyma bogoriensis glucosyltransferase II UDP + 13-[(2'-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]docosanoic acid methyl ester
-
 ?
2.4.1.158 UDPglucose + 13-hydroxydocosanoic acid Pseudohyphozyma bogoriensis glucosyltransferase I UDP + 13-(beta-D-glucopyranosyl)oxydocosanoic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.158 Pseudohyphozyma bogoriensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.158 partial Pseudohyphozyma bogoriensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.158 0.0222
-
 glucosyltransferase I Pseudohyphozyma bogoriensis
2.4.1.158 0.0242
-
 glucosyltransferase II Pseudohyphozyma bogoriensis

Storage Stability

EC Number Storage Stability Organism
2.4.1.158 4°C, 20% glycerol: half-life 35 days Pseudohyphozyma bogoriensis
2.4.1.158 4°C, without glycerol: half-life 5 days Pseudohyphozyma bogoriensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.158 additional information enzyme seems to be a single multifunctional protein catalyzing both transferase reactions Pseudohyphozyma bogoriensis ?
-
 ?
2.4.1.158 UDPglucose + 13-(beta-D-glucopyranosyl)oxydocosanoic acid methyl ester glucosyltransferase II Pseudohyphozyma bogoriensis UDP + 13-[(2'-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]docosanoic acid methyl ester
-
 ?
2.4.1.158 UDPglucose + 13-hydroxydocosanoic acid glucosyltransferase I Pseudohyphozyma bogoriensis UDP + 13-(beta-D-glucopyranosyl)oxydocosanoic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.158 monomer 1 * 52000, SDS-PAGE Pseudohyphozyma bogoriensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.158 25
-
 assay at Pseudohyphozyma bogoriensis
2.4.1.158 30 37
-
 Pseudohyphozyma bogoriensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.158 5 37 30°C-37°C: 100% of activity, at 5°C: 10% of maximal activity Pseudohyphozyma bogoriensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.158 6.5 8 glucosyltransferase I, within this range activity of glucosyltransferase II increases Pseudohyphozyma bogoriensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.158 5.9 8.1 glucosyltransferase I and II: stable Pseudohyphozyma bogoriensis