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Literature summary extracted from

  • Jang, K.H.; Song, K.B.; Kim, C.H.; Chung, B.H.; Kang, S.A.; Chun, U.H.; Choue, R.W.; Rhee, S.K.
    Comparison of characteristics of levan produced by different preparations of levansucrase from Zymomonas mobilis (2001), Biotechnol. Lett., 23, 339-344.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.4.1.10 synthesis immobilization of recombinant free levansucrase on magnetite leads to production of low molecular weight levan, increased thermal stability of the enzyme Zymomonas mobilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.10 expression as His-tagged protein in Escherichia coli BL21(DE3), genel evU Zymomonas mobilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.10 additional information Zymomonas mobilis production of low molecular weight levan in permeabilized recombinant E. coli cells ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Zymomonas mobilis Q60114 gene levU
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Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.10 recombinant from E. coli as His-tagged protein Zymomonas mobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 additional information production of low molecular weight levan in permeabilized recombinant E. coli cells Zymomonas mobilis ?
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 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n
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 Zymomonas mobilis glucose + (2,6-beta-D-fructosyl)n+1 molecular weight of produced levan is dependent on preparation method, immobilized recombinant enzyme and recombinant enzyme in permeabilized E. coli cells produces low molecular weight levan, free recombinant enzyme produces high molecular weight levan ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.10 additional information
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 at lower temperatures, such as 4°C and 15°C, the transfructosylation is preferentially catalyzed rather than the hydrolysis of sucrose, but inversely at higher temperatures such as 30°C and 40°C the hydrolysis is preferentially catalyzed Zymomonas mobilis