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Literature summary extracted from

  • Matheson, N.K.; Richardson, R.H.
    Kinetic properties of two starch phosphorylases from pea seeds (1973), Phytochemistry, 17, 195-200.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 ADPglucose 1.3 mM, 50% inhibition of phosphorylase II, glucan phosphorolysis; strong inhibition of phosphorolysis, isozyme I Pisum sativum
2.4.1.1 deoxypyridoxine weak inhibition of phosphorylase I, not II Pisum sativum
2.4.1.1 additional information not inhibited by fructose 6-phosphate Pisum sativum
2.4.1.1 pyridine 3-aldehyde weak inhibition of phosphorylase I, not II Pisum sativum
2.4.1.1 pyridine 4-aldehyde inhibition of phosphorylase I, not II Pisum sativum
2.4.1.1 pyridoxal phosphorylase I, not II Pisum sativum
2.4.1.1 pyridoxal 5'-phosphate 74 and 76% inhibition of phosphorylase II and I respectively Pisum sativum
2.4.1.1 pyridoxamine weak inhibition of phosphorylase I, not II Pisum sativum
2.4.1.1 pyridoxamine 5'-phosphate weak inhibition of phosphorylase I, not II Pisum sativum
2.4.1.1 UDPglucose 2-3 mM, 50% inhibition of phosphorylase II, glucan phosphorolysis Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
 additional information kinetic data Pisum sativum
2.4.1.1 4
-
 glucose 1-phosphate phosphorylase I Pisum sativum
2.4.1.1 5.5
-
 glucose 1-phosphate phosphorylase II Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Pisum sativum
-
 Victory Freezer
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 phosphorylase I, starch-Sepharose column, DEAE-cellulose, phosphorylase II, DEAE-cellulose, starch-Sepharose Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 seed
-
 Pisum sativum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 amylopectin + glucose 1-phosphate potato amylopectin Pisum sativum amylopectin + phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Pisum sativum [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.1.1 pyridoxal 5'-phosphate requirement Pisum sativum
2.4.1.1 pyridoxal 5'-phosphate additional pyridoxalphosphate inhibits Pisum sativum