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Literature summary extracted from

  • Iwata, T.; Funagama, T.; Hara, A.
    Purification and some properties of two phosphorylases from Typha latifolia pollen (1988), Agric. Biol. Chem., 52, 407-412.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
 additional information kinetic data Typha latifolia
2.4.1.1 0.12
-
 maltoheptaose synthesis, isoenzyme P-2 Typha latifolia
2.4.1.1 0.18
-
 maltoheptaose synthesis, isoenzyme P-2 Typha latifolia
2.4.1.1 0.26
-
 maltoheptaose synthesis, isoenzyme P-1 Typha latifolia
2.4.1.1 0.49
-
 maltopentaose synthesis, isoenzyme P-2 Typha latifolia
2.4.1.1 0.59
-
 maltoheptaose synthesis, isoenzyme P-1 Typha latifolia
2.4.1.1 0.88
-
 maltotriose synthesis, isoenzyme P-2 Typha latifolia
2.4.1.1 1.1
-
 maltoheptaose synthesis, isoenzyme P-1 Typha latifolia
2.4.1.1 1.7
-
 maltopentaose synthesis, isoenzyme P-1 Typha latifolia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 112000
-
 x * 112000, SDS-PAGE Typha latifolia

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Typha latifolia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 isozymes P-1 and P-2, ammonium sulfate, DE-52, amylose-Sepharose, BioGel Typha latifolia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 pollen grain mature Typha latifolia
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 11
-
 isozyme P-2 Typha latifolia
2.4.1.1 42.3
-
 isozyme P-1 Typha latifolia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 amylose + glucose 1-phosphate
-
 Typha latifolia amylose + phosphate
-
 r
2.4.1.1 maltoheptaose + glucose 1-phosphate isozyme I Typha latifolia maltooctaose + phosphate
-
 r
2.4.1.1 maltopentaose + glucose 1-phosphate isozyme I Typha latifolia maltohexaose + phosphate
-
 r
2.4.1.1 maltotriose + alpha-D-glucose 1-phosphate
-
 Typha latifolia maltotetraose + phosphate
-
 r
2.4.1.1 starch + glucose 1-phosphate soluble starch Typha latifolia starch + phosphate
-
 r
2.4.1.1 starch + glucose 1-phosphate glucosylation of native starch granules occurs at 82%, isozyme P-1, or 11.5%, isozyme P-2, the rate of soluble starch, phosphorolysis at the same rate as soluble starch Typha latifolia starch + phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Typha latifolia [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.1 ? x * 112000, SDS-PAGE Typha latifolia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 5.5 5.8 isoenzymes P-1 and P-2, phosphorolysis Typha latifolia
2.4.1.1 7.3 8 synthesis Typha latifolia