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Literature summary extracted from

  • Sambandam, T.; Gunasekaran, M.
    Purification and properties of phosphorylase from Phymatotrichum omnivorum (1987), Arch. Biochem. Biophys., 254, 579-585.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 2,2'-dipyridyl
-
 Phymatotrichopsis omnivora
2.4.1.1 ADP
-
 Phymatotrichopsis omnivora
2.4.1.1 ATP
-
 Phymatotrichopsis omnivora
2.4.1.1 Ba2+
-
 Phymatotrichopsis omnivora
2.4.1.1 Ca2+ 1 mM, stimulation at 10 mM Phymatotrichopsis omnivora
2.4.1.1 Co2+ 1 mM, no inhibition at 10 mM Phymatotrichopsis omnivora
2.4.1.1 Cu2+ 10 mM, complete inhibition Phymatotrichopsis omnivora
2.4.1.1 cysteine 1 mM, 86% inhibition Phymatotrichopsis omnivora
2.4.1.1 diethyldithiocarbamate 1 mM, 94% inhibition Phymatotrichopsis omnivora
2.4.1.1 dithiothreitol 1 mM, 56% inhibition Phymatotrichopsis omnivora
2.4.1.1 EDTA 1 mM, 61% inhibition Phymatotrichopsis omnivora
2.4.1.1 EGTA i.e. ethylene glycol bis(beta-aminoethylether)-N,N'-tetraacetic acid, 1 mM, 53% inhibition Phymatotrichopsis omnivora
2.4.1.1 Fe2+ 10 mM, stimulation at 1 mM Phymatotrichopsis omnivora
2.4.1.1 glutathione
-
 Phymatotrichopsis omnivora
2.4.1.1 iodoacetate weak inhibition Phymatotrichopsis omnivora
2.4.1.1 Mn2+
-
 Phymatotrichopsis omnivora
2.4.1.1 Zn2+ strong inhibition Phymatotrichopsis omnivora

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
 additional information 0.36 mg/ml, glycogen Phymatotrichopsis omnivora

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.1 cytoplasm
-
 Phymatotrichopsis omnivora 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.1 Ca2+ stimulates at 10 mM, inhibits at 1 mM Phymatotrichopsis omnivora
2.4.1.1 Fe2+ stimulates at 1 mM, inhibits at 10 mM Phymatotrichopsis omnivora

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 119000
-
 gel filtration Phymatotrichopsis omnivora
2.4.1.1 145000
-
 x * 145000, SDS-PAGE Phymatotrichopsis omnivora

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Phymatotrichopsis omnivora
-
-
-
2.4.1.1 Phymatotrichopsis omnivora Shear Dugg
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 ammonium sulfate, Sephacryl S-200, DEAE-cellulose Phymatotrichopsis omnivora

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 mycelium
-
 Phymatotrichopsis omnivora
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 222
-
-
 Phymatotrichopsis omnivora

Storage Stability

EC Number Storage Stability Organism
2.4.1.1 4°C, 30 days, 20% loss of activity Phymatotrichopsis omnivora

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 (dextrin)n-1 + glucose 1-phosphate corn dextrin, phosphorolysis at 85% the rate of glycogen phosphorolysis Phymatotrichopsis omnivora (dextrin)n + phosphate
-
 r
2.4.1.1 (dextrin)n-1 + glucose 1-phosphate corn dextrin, phosphorolysis at 85% the rate of glycogen phosphorolysis Phymatotrichopsis omnivora Shear Dugg (dextrin)n + phosphate
-
 r
2.4.1.1 glycogen + glucose 1-phosphate best substrate Phymatotrichopsis omnivora glycogen + phosphate
-
 ?
2.4.1.1 glycogen + glucose 1-phosphate best substrate Phymatotrichopsis omnivora Shear Dugg glycogen + phosphate
-
 ?
2.4.1.1 starch + glucose 1-phosphate phosphorolysis at 31% the rate of glycogen phosphorolysis Phymatotrichopsis omnivora starch + phosphate
-
 r
2.4.1.1 starch + glucose 1-phosphate phosphorolysis at 31% the rate of glycogen phosphorolysis Phymatotrichopsis omnivora Shear Dugg starch + phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Phymatotrichopsis omnivora [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Phymatotrichopsis omnivora Shear Dugg [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.1 ? x * 145000, SDS-PAGE Phymatotrichopsis omnivora

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.1 30
-
 15 min, slight stimulation Phymatotrichopsis omnivora
2.4.1.1 40
-
 15 min, 16% loss of activity Phymatotrichopsis omnivora
2.4.1.1 70
-
 15 min, complete inactivation Phymatotrichopsis omnivora

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 7
-
-
 Phymatotrichopsis omnivora

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.1 5.6 8 approx. half-maximal activity at pH 5.6 and approx. 65% of maximal activity at pH 8, citrate/phosphate-buffer Phymatotrichopsis omnivora
2.4.1.1 6 8.6 approx. 70% of maximal activity at pH 6 and about half-maximal activity at pH 8.6, Tris-buffer Phymatotrichopsis omnivora

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.1 4 9 stable Phymatotrichopsis omnivora