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Literature summary extracted from

  • Muir, J.M.; Russell, R.J.M.; Hough, D.W.; Danson, M.J.
    Citrate synthase from the hyperthermophilic archaeon, Pyrococcus furiosus (1995), Protein Eng., 8, 583-592.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.16 overexpression in Escherichia coli JM105, DNA and amino acid sequence analysis Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 0.0017
-
 acetyl-CoA recombinant enzyme Pyrococcus furiosus
2.3.3.16 0.005
-
 oxaloacetate recombinant enzyme Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.16 KCl 5fold increase at 100-200 mM Pyrococcus furiosus
2.3.3.16 NaCl 5fold increase at 100-200 mM Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.16 additional information
-
 amino acid sequence alignment Pyrococcus furiosus
2.3.3.16 additional information
-
 secondary and tertiary structure Pyrococcus furiosus
2.3.3.16 42600
-
 2 * 42600, SDS-PAGE Pyrococcus furiosus
2.3.3.16 89700
-
 gel filtration Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16 recombinant enzyme from E. coli Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.16 30
-
 purified recombinant enzyme from E. coli Pyrococcus furiosus
2.3.3.16 112.5
-
 recombinant enzyme Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Pyrococcus furiosus citrate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.16 dimer 2 * 42600, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.16 55
-
 enzyme assay at Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.3.16 100
-
 half-life: 20 min Pyrococcus furiosus
2.3.3.16 104
-
 half-life: 1 min Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.16 8
-
 enzyme assay at Pyrococcus furiosus