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Literature summary extracted from

  • Grossebuter, W.; Goerisch, H.
    Partial purification and properties of citrate synthase from the thermoacidophilic archaebacteria Thermoplasma acidophilum and Sulfolobus acidocaldarius (1985), Syst. Appl. Microbiol., 6, 119-124.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 ATP competitive against acetyl-CoA Sulfolobus acidocaldarius
2.3.3.16 ATP competitive against acetyl-CoA Thermoplasma acidophilum
2.3.3.16 NADH competitive against acetyl-CoA Sulfolobus acidocaldarius
2.3.3.16 NADH competitive against acetyl-CoA Thermoplasma acidophilum
2.3.3.16 NADPH competitive against acetyl-CoA Sulfolobus acidocaldarius
2.3.3.16 NADPH competitive against acetyl-CoA Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 0.0041
-
 oxaloacetate
-
 Thermoplasma acidophilum
2.3.3.16 0.006
-
 acetyl-CoA
-
 Thermoplasma acidophilum
2.3.3.16 0.01
-
 acetyl-CoA
-
 Sulfolobus acidocaldarius
2.3.3.16 0.026
-
 oxaloacetate
-
 Sulfolobus acidocaldarius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.16 68000
-
 gel filtration Thermoplasma acidophilum
2.3.3.16 73000
-
 gel filtration Sulfolobus acidocaldarius

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Sulfolobus acidocaldarius
-
-
-
2.3.3.16 Sulfolobus acidocaldarius 98-3 / DSM 639
-
-
-
2.3.3.16 Thermoplasma acidophilum
-
-
-
2.3.3.16 Thermoplasma acidophilum 122-1B3 / ATCC 27658
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16 partial Sulfolobus acidocaldarius
2.3.3.16 partial Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.16 14
-
 partially purified enzyme Sulfolobus acidocaldarius
2.3.3.16 18.8
-
 partially purified enzyme Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Sulfolobus acidocaldarius citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Thermoplasma acidophilum citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Sulfolobus acidocaldarius 98-3 / DSM 639 citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Thermoplasma acidophilum 122-1B3 / ATCC 27658 citrate + CoA
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.3.16 80
-
 90% of activity after 10 min Sulfolobus acidocaldarius
2.3.3.16 80
-
 65% of activity retained after 10 min Thermoplasma acidophilum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.16 0.4
-
 ATP
-
 Sulfolobus acidocaldarius
2.3.3.16 0.5
-
 ATP
-
 Thermoplasma acidophilum
2.3.3.16 0.8
-
 NADPH
-
 Sulfolobus acidocaldarius
2.3.3.16 4.4
-
 NADH
-
 Sulfolobus acidocaldarius
2.3.3.16 4.9
-
 NADPH
-
 Thermoplasma acidophilum
2.3.3.16 10.4
-
 NADH
-
 Thermoplasma acidophilum