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Literature summary extracted from

  • Gräwe, W.; Strack, D.
    Partial purification and some properties of 1-sinapoylglucose: choline sinapoyltransferase (sinapine synthase) from seeds of Raphanus sativus L. and Sinapis alba L. (1986), Z. Naturforsch. C, 41, 28-33.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.91 Ca2+ strong, above 5 mM Raphanus sativus
2.3.1.91 Ca2+ strong, above 5 mM Sinapis alba
2.3.1.91 Co2+ strong, above 5 mM Raphanus sativus
2.3.1.91 Co2+ strong, above 5 mM Sinapis alba
2.3.1.91 dithioerythritol
-
 Raphanus sativus
2.3.1.91 dithioerythritol
-
 Sinapis alba
2.3.1.91 dithiothreitol
-
 Raphanus sativus
2.3.1.91 dithiothreitol
-
 Sinapis alba
2.3.1.91 EDTA
-
 Raphanus sativus
2.3.1.91 EDTA
-
 Sinapis alba
2.3.1.91 Mn2+ strong, above 5 mM Raphanus sativus
2.3.1.91 Mn2+ strong, above 5 mM Sinapis alba
2.3.1.91 SDS
-
 Raphanus sativus
2.3.1.91 SDS
-
 Sinapis alba

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.91 0.11
-
 1,2-Di-O-sinapoyl-beta-D-glucose
-
 Raphanus sativus
2.3.1.91 0.2
-
 1,2-Di-O-sinapoyl-beta-D-glucose
-
 Sinapis alba
2.3.1.91 0.48
-
 1-O-sinapoyl-beta-D-glucose
-
 Raphanus sativus
2.3.1.91 0.71
-
 1-O-sinapoyl-beta-D-glucose
-
 Sinapis alba
2.3.1.91 5.3
-
 choline
-
 Raphanus sativus
2.3.1.91 6.5
-
 choline
-
 Sinapis alba

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.91 additional information no requirement for divalent cations Raphanus sativus
2.3.1.91 additional information no requirement for divalent cations Sinapis alba

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.91 60000
-
 gel filtration Raphanus sativus
2.3.1.91 60000
-
 gel filtration Sinapis alba

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.91 1-O-sinapoyl-beta-D-glucose + choline Raphanus sativus involved in sinapine biosynthesis D-glucose + sinapoylcholine
-
 ?
2.3.1.91 1-O-sinapoyl-beta-D-glucose + choline Sinapis alba involved in sinapine biosynthesis D-glucose + sinapoylcholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.91 Raphanus sativus
-
 var. sativus cv. Saxa
-
2.3.1.91 Sinapis alba
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.91 partially Raphanus sativus
2.3.1.91 partially Sinapis alba

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.91 seed immature dark-green Raphanus sativus
-
2.3.1.91 seed immature dark-green Sinapis alba
-
2.3.1.91 seed 20% activity in mature seeds compared to immature seeds Raphanus sativus
-
2.3.1.91 seed 20% activity in mature seeds compared to immature seeds Sinapis alba
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.91 0.258
-
 partially purified enzyme Sinapis alba
2.3.1.91 0.486
-
 partially purified enzyme Raphanus sativus

Storage Stability

EC Number Storage Stability Organism
2.3.1.91 -20°C, crude extract, 1 week, loss of 20% activity Raphanus sativus
2.3.1.91 -20°C, crude extract, 1 week, loss of 20% activity Sinapis alba
2.3.1.91 -20°C, partially purified, 2 weeks upon repeated freezing and thawing Raphanus sativus
2.3.1.91 -20°C, partially purified, 2 weeks upon repeated freezing and thawing Sinapis alba

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.91 1,2-di-O-sinapoyl-beta-D-glucose + choline best donor substrate Raphanus sativus 2-O-sinapoyl-beta-D-glucose + sinapoylcholine
-
 ?
2.3.1.91 1,2-di-O-sinapoyl-beta-D-glucose + choline best donor substrate Sinapis alba 2-O-sinapoyl-beta-D-glucose + sinapoylcholine
-
 ?
2.3.1.91 1-feruloyl-beta-D-glucose + choline
-
 Raphanus sativus D-glucose + feruloylcholine
-
 ?
2.3.1.91 1-feruloyl-beta-D-glucose + choline
-
 Sinapis alba D-glucose + feruloylcholine
-
 ?
2.3.1.91 1-O-sinapoyl-beta-D-glucose + choline no donors are 1-benzoylglucose, 1-galloylglucose, 6-sinapoylglucose, 3-sinapoylfructose Raphanus sativus D-glucose + sinapoylcholine i.e. sinapine ?
2.3.1.91 1-O-sinapoyl-beta-D-glucose + choline no donors are 1-benzoylglucose, 1-galloylglucose, 6-sinapoylglucose, 3-sinapoylfructose Sinapis alba D-glucose + sinapoylcholine i.e. sinapine ?
2.3.1.91 1-O-sinapoyl-beta-D-glucose + choline absolute acceptor specificity towards choline Raphanus sativus D-glucose + sinapoylcholine i.e. sinapine ?
2.3.1.91 1-O-sinapoyl-beta-D-glucose + choline absolute acceptor specificity towards choline Sinapis alba D-glucose + sinapoylcholine i.e. sinapine ?
2.3.1.91 1-O-sinapoyl-beta-D-glucose + choline involved in sinapine biosynthesis Raphanus sativus D-glucose + sinapoylcholine
-
 ?
2.3.1.91 1-O-sinapoyl-beta-D-glucose + choline involved in sinapine biosynthesis Sinapis alba D-glucose + sinapoylcholine
-
 ?
2.3.1.91 1-p-coumaroyl-beta-D-glucose + choline
-
 Sinapis alba D-glucose + p-coumaroylcholine
-
 ?
2.3.1.91 1-p-coumaroyl-beta-D-glucose + choline poor substrate Raphanus sativus D-glucose + p-coumaroylcholine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.91 SCT
-
 Raphanus sativus
2.3.1.91 SCT
-
 Sinapis alba

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.91 45
-
-
 Raphanus sativus
2.3.1.91 45
-
-
 Sinapis alba

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.91 7.2
-
-
 Raphanus sativus
2.3.1.91 7.6
-
-
 Sinapis alba

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.91 5.5 10
-
 Raphanus sativus
2.3.1.91 5.5 10
-
 Sinapis alba