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Literature summary extracted from

  • Dittrich, F.; Zajonc, D.; Huhne, K.; Hoja, U.; Ekici, A.; Greiner, E.; Klein, H.; Hofmann, J.; Bessoule, J.J.; Sperling, P.; Schweizer, E.
    Fatty acid elongation in yeast. Biochemical characteristics of the enzyme system and isolation of elongation-defective mutants (1998), Eur. J. Biochem., 252, 477-485.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.86 ATP stimulatory at suboptimal but not at saturating substrate concentrations Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.86
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.86 0.05
-
 lauroyl-CoA
-
 Saccharomyces cerevisiae
2.3.1.86 0.13
-
 palmitoyl-CoA
-
 Saccharomyces cerevisiae
2.3.1.86 0.33
-
 octanoyl-CoA
-
 Saccharomyces cerevisiae
2.3.1.86 0.4
-
 myristoyl-CoA
-
 Saccharomyces cerevisiae
2.3.1.86 0.83
-
 decanoyl-CoA
-
 Saccharomyces cerevisiae
2.3.1.199 0.05
-
 lauroyl-CoA in 10 mM Tris/maleic acid pH 6.7, 0.65 M mannitol and 0.36 mM EGTA, at 30°C Saccharomyces cerevisiae
2.3.1.199 0.13
-
 palmitoyl-CoA in 10 mM Tris/maleic acid pH 6.7, 0.65 M mannitol and 0.36 mM EGTA, at 30°C Saccharomyces cerevisiae
2.3.1.199 0.33
-
 octanoyl-CoA in 10 mM Tris/maleic acid pH 6.7, 0.65 M mannitol and 0.36 mM EGTA, at 30°C Saccharomyces cerevisiae
2.3.1.199 0.4
-
 myristoyl-CoA in 10 mM Tris/maleic acid pH 6.7, 0.65 M mannitol and 0.36 mM EGTA, at 30°C Saccharomyces cerevisiae
2.3.1.199 0.83
-
 decanoyl-CoA in 10 mM Tris/maleic acid pH 6.7, 0.65 M mannitol and 0.36 mM EGTA, at 30°C Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.199 membrane
-
 Saccharomyces cerevisiae 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.199 32000
-
 x * 32000, SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.86 Saccharomyces cerevisiae
-
 mutants lacking endogenous de novo fatty acid synthesis
-
2.3.1.199 Saccharomyces cerevisiae
-
-
-
2.3.1.199 Saccharomyces cerevisiae X2180-1A
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.86 acetyl-CoA + 7 malonyl-CoA + 7 NADH + 7 NADPH + 14 H+ mutants require acyl-CoA primers of 10 or more carbon atoms, maximal activity with 12-14 carbon atoms Saccharomyces cerevisiae palmitoyl-CoA + 7 CoA + 7 CO2 + 7 NAD+ + 7 NADP+ + 7 H2O
-
 ?
2.3.1.86 acetyl-CoA + 7 malonyl-CoA + 7 NADH + 7 NADPH + 14 H+ mutants require acyl-CoA primers of 10 or more carbon atoms, maximal activity with 12-14 carbon atoms Saccharomyces cerevisiae X2180-1A palmitoyl-CoA + 7 CoA + 7 CO2 + 7 NAD+ + 7 NADP+ + 7 H2O
-
 ?
2.3.1.86 decanoyl-CoA + malonyl-CoA + NADPH + H+
-
 Saccharomyces cerevisiae ?
-
 ?
2.3.1.86 decanoyl-CoA + malonyl-CoA + NADPH + H+
-
 Saccharomyces cerevisiae X2180-1A ?
-
 ?
2.3.1.86 lauroyl-CoA + malonyl-CoA + NADPH + H+
-
 Saccharomyces cerevisiae ?
-
 ?
2.3.1.86 lauroyl-CoA + malonyl-CoA + NADPH + H+
-
 Saccharomyces cerevisiae X2180-1A ?
-
 ?
2.3.1.86 myristoyl-CoA + malonyl-CoA + NADPH + H+
-
 Saccharomyces cerevisiae ?
-
 ?
2.3.1.86 myristoyl-CoA + malonyl-CoA + NADPH + H+
-
 Saccharomyces cerevisiae X2180-1A ?
-
 ?
2.3.1.86 octanoyl-CoA + malonyl-CoA + NADPH + H+
-
 Saccharomyces cerevisiae ?
-
 ?
2.3.1.86 octanoyl-CoA + malonyl-CoA + NADPH + H+
-
 Saccharomyces cerevisiae X2180-1A ?
-
 ?
2.3.1.199 decanoyl-CoA + malonyl-CoA
-
 Saccharomyces cerevisiae ?
-
 ?
2.3.1.199 lauroyl-CoA + malonyl-CoA
-
 Saccharomyces cerevisiae ?
-
 ?
2.3.1.199 myristoyl-CoA + malonyl-CoA best substrate Saccharomyces cerevisiae ?
-
 ?
2.3.1.199 octanoyl-CoA + malonyl-CoA
-
 Saccharomyces cerevisiae ?
-
 ?
2.3.1.199 palmitoyl-CoA + malonyl-CoA
-
 Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.199 ? x * 32000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.3.1.199 acyl-CoA elongase
-
 Saccharomyces cerevisiae
2.3.1.199 ELo1
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.86 NADPH
-
 Saccharomyces cerevisiae