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Literature summary extracted from

  • Sutfeld, R.; Kehrel, B.; Wiermann, R.
    Characterization and development and localization of Flavanone synthase in tulip anthers (1978), Z. Naturforsch. C, 33c, 841-846.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.74 2-mercaptoethanol activation Tulipa hybrid cultivar

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.74 2-mercaptoethanol above 5 mM Tulipa hybrid cultivar
2.3.1.74 Ca2+ 10-20% decrease at 1 mM Tulipa hybrid cultivar
2.3.1.74 CoA total inhibition at 0.25 mM Tulipa hybrid cultivar
2.3.1.74 Cu2+ 50% decrease above 1 mM Tulipa hybrid cultivar
2.3.1.74 dithiothreitol 60% inhibition Tulipa hybrid cultivar
2.3.1.74 Mg2+ 10-20% decrease at 1 mM Tulipa hybrid cultivar
2.3.1.74 naringenin total inhibition at 0.4 mM Tulipa hybrid cultivar
2.3.1.74 p-chloromercuribenzoate 50% inhibition at 2.5 mM Tulipa hybrid cultivar
2.3.1.74 Zn2+ 50% decrease above 1 mM Tulipa hybrid cultivar

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.74 0.0016
-
 caffeoyl-CoA
-
 Tulipa hybrid cultivar
2.3.1.74 0.0017
-
 4-coumaroyl-CoA
-
 Tulipa hybrid cultivar
2.3.1.74 0.0025
-
 feruloyl-CoA
-
 Tulipa hybrid cultivar

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.74 55000
-
 gel filtration, gradient centrifugation Tulipa hybrid cultivar

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.74 Tulipa hybrid cultivar
-
 cv. Apeldoorn
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.74 partial Tulipa hybrid cultivar

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.74 anther
-
 Tulipa hybrid cultivar
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.74 additional information
-
-
 Tulipa hybrid cultivar

Storage Stability

EC Number Storage Stability Organism
2.3.1.74 -20°C, 30% initial loss of activity, remaining activity stable for 14 d Tulipa hybrid cultivar

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.74 caffeoyl-CoA + 3 malonyl-CoA
-
 Tulipa hybrid cultivar eriodictyol + 4 CoA + 3 CO2
-
 ?
2.3.1.74 ferulyl-CoA + malonyl-CoA
-
 Tulipa hybrid cultivar homoeriodictyol + CoA + CO2
-
 ?
2.3.1.74 malonyl-CoA + 4-coumaroyl-CoA
-
 Tulipa hybrid cultivar naringenin chalcone + CoA + CO2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.74 30
-
-
 Tulipa hybrid cultivar

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.74 20 50 less than 50% of maximal activity above and below Tulipa hybrid cultivar
2.3.1.74 20 60 50% activity at 20°C, 50°C and 60°C Tulipa hybrid cultivar

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.74 25
-
 50% inactivation after 90 min Tulipa hybrid cultivar

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.74 8
-
-
 Tulipa hybrid cultivar