BRENDA - Enzyme Database

Metabolism of monoterpenes. Acetylation of (-)-menthol by a soluble enzyme preparation from peppermint (Mentha piperita) leaves

Croteau, R.; Hooper, C.L.; Plant Physiol. 61, 737-742 (1978) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.69
dithioerythritol
required for maximal activity
Mentha x piperita
General Stability
EC Number
General Stability
Organism
2.3.1.69
dithioerythritol, required for maximal stability
Mentha x piperita
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.69
diisopropylfluorophosphate
strong inhibitor, 50% inhibition at 0.1 mM
Mentha x piperita
2.3.1.69
EDTA
slightly inhibitory
Mentha x piperita
2.3.1.69
p-hydroxymercuribenzoate
-
Mentha x piperita
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.69
0.2
-
(-)-menthol
flower head enzyme
Mentha x piperita
2.3.1.69
0.3
-
(-)-menthol
leaf enzyme
Mentha x piperita
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.69
soluble
100000 g supernatant fraction
Mentha x piperita
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.69
37000
-
gel filtration
Mentha x piperita
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.1.69
Mentha x piperita
-
peppermint
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.3.1.69
partial
Mentha x piperita
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.69
flower
-
Mentha x piperita
-
2.3.1.69
leaf
-
Mentha x piperita
-
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.69
0°C, 5 mM dithioerythritol, 15% glycerol, 60% loss of activity after 24 h
Mentha x piperita
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.1.69
(+)-2-terpineol + acetyl-CoA
low activity
487396
Mentha x piperita
CoA + (+)-2-terpineol acetate
-
-
-
?
2.3.1.69
(+)-isomenthol + acetyl-CoA
-
487396
Mentha x piperita
CoA + (+)-isomenthol acetate
-
-
-
?
2.3.1.69
(+)-menthol + acetyl-CoA
-
487396
Mentha x piperita
CoA + (+)-menthol acetate
-
-
-
?
2.3.1.69
(+)-neoisomenthol + acetyl-CoA
-
487396
Mentha x piperita
CoA + (+)-neoisomenthol acetate
-
-
-
?
2.3.1.69
(-)-isomenthol + acetyl-CoA
-
487396
Mentha x piperita
CoA + (-)-isomenthol acetate
-
-
-
?
2.3.1.69
(-)-neoisomenthol + acetyl-CoA
-
487396
Mentha x piperita
CoA + (-)-neoisomenthol acetate
-
-
-
?
2.3.1.69
(-)-neomenthol + acetyl-CoA
-
487396
Mentha x piperita
CoA + (-)-neomenthol acetate
-
-
-
?
2.3.1.69
acetyl-CoA + (+)-neomenthol
-
487396
Mentha x piperita
CoA + neomenthyl acetate
-
-
-
?
2.3.1.69
acetyl-CoA + (-)-menthol
-
487396
Mentha x piperita
CoA + (-)-menthyl acetate
-
487396
Mentha x piperita
?
2.3.1.69
acetyl-CoA + borneol
-
487396
Mentha x piperita
CoA + borneol acetate
-
-
-
?
2.3.1.69
acetyl-CoA + cyclohexanol
-
487396
Mentha x piperita
CoA + cyclohexanol acetate
-
-
-
?
2.3.1.69
acetyl-CoA + n-decanol
best substrate
487396
Mentha x piperita
CoA + n-decanol acetate
-
-
-
?
2.3.1.69
additional information
specific for acetyl-CoA, propionyl-CoA reacts with 11% of the activity of acetyl-CoA, butyryl-CoA reacts with 2% of the activity of acetyl-CoA, enzyme has a slight preference for stereomers in which the hydroxyl and isopropyl are cis located
487396
Mentha x piperita
?
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.69
9
-
-
Mentha x piperita
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.69
7.5
10
pH 7.5: about 50% of activity maximum, increase of activity up to pH 9.5, pH 10: about 40% of activity maximum
Mentha x piperita
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.69
dithioerythritol
required for maximal activity
Mentha x piperita
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.69
dithioerythritol, required for maximal stability
Mentha x piperita
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.69
diisopropylfluorophosphate
strong inhibitor, 50% inhibition at 0.1 mM
Mentha x piperita
2.3.1.69
EDTA
slightly inhibitory
Mentha x piperita
2.3.1.69
p-hydroxymercuribenzoate
-
Mentha x piperita
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.69
0.2
-
(-)-menthol
flower head enzyme
Mentha x piperita
2.3.1.69
0.3
-
(-)-menthol
leaf enzyme
Mentha x piperita
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.69
soluble
100000 g supernatant fraction
Mentha x piperita
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.69
37000
-
gel filtration
Mentha x piperita
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.69
partial
Mentha x piperita
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.69
flower
-
Mentha x piperita
-
2.3.1.69
leaf
-
Mentha x piperita
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.69
0°C, 5 mM dithioerythritol, 15% glycerol, 60% loss of activity after 24 h
Mentha x piperita
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.1.69
(+)-2-terpineol + acetyl-CoA
low activity
487396
Mentha x piperita
CoA + (+)-2-terpineol acetate
-
-
-
?
2.3.1.69
(+)-isomenthol + acetyl-CoA
-
487396
Mentha x piperita
CoA + (+)-isomenthol acetate
-
-
-
?
2.3.1.69
(+)-menthol + acetyl-CoA
-
487396
Mentha x piperita
CoA + (+)-menthol acetate
-
-
-
?
2.3.1.69
(+)-neoisomenthol + acetyl-CoA
-
487396
Mentha x piperita
CoA + (+)-neoisomenthol acetate
-
-
-
?
2.3.1.69
(-)-isomenthol + acetyl-CoA
-
487396
Mentha x piperita
CoA + (-)-isomenthol acetate
-
-
-
?
2.3.1.69
(-)-neoisomenthol + acetyl-CoA
-
487396
Mentha x piperita
CoA + (-)-neoisomenthol acetate
-
-
-
?
2.3.1.69
(-)-neomenthol + acetyl-CoA
-
487396
Mentha x piperita
CoA + (-)-neomenthol acetate
-
-
-
?
2.3.1.69
acetyl-CoA + (+)-neomenthol
-
487396
Mentha x piperita
CoA + neomenthyl acetate
-
-
-
?
2.3.1.69
acetyl-CoA + (-)-menthol
-
487396
Mentha x piperita
CoA + (-)-menthyl acetate
-
487396
Mentha x piperita
?
2.3.1.69
acetyl-CoA + borneol
-
487396
Mentha x piperita
CoA + borneol acetate
-
-
-
?
2.3.1.69
acetyl-CoA + cyclohexanol
-
487396
Mentha x piperita
CoA + cyclohexanol acetate
-
-
-
?
2.3.1.69
acetyl-CoA + n-decanol
best substrate
487396
Mentha x piperita
CoA + n-decanol acetate
-
-
-
?
2.3.1.69
additional information
specific for acetyl-CoA, propionyl-CoA reacts with 11% of the activity of acetyl-CoA, butyryl-CoA reacts with 2% of the activity of acetyl-CoA, enzyme has a slight preference for stereomers in which the hydroxyl and isopropyl are cis located
487396
Mentha x piperita
?
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.69
9
-
-
Mentha x piperita
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.69
7.5
10
pH 7.5: about 50% of activity maximum, increase of activity up to pH 9.5, pH 10: about 40% of activity maximum
Mentha x piperita