Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Falany, C.N.; Fortinberry, H.; Leiter, E.H.; Barnes, S.
    Cloning, expression, and chromosomal localization of mouse liver bile acid CoA:amino acid N-acyltransferase (1997), J. Lipid Res., 38, 1139-1148.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.65 cDNA encoding enzyme is cloned, sequenced and expressed in Escherichia coli DH5alpha, the open-reading frame codes for a 420 amino acid protein, Baat gene is mapped to mouse chromosome 4, single gene encodes enzyme Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.65 additional information not inhibited by glycine Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.65 1.9
-
 taurine recombinant enzyme, expressed in Escherichia coli Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.65 45000
-
 x * 46525, calculated from the amino acid sequence, x * 45000, recombinant enzyme, SDS-PAGE Mus musculus
2.3.1.65 46525
-
 x * 46525, calculated from the amino acid sequence, x * 45000, recombinant enzyme, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.65 choloyl-CoA + taurine Mus musculus taurine-specific enzyme, gallbladder bile contains only taurine conjugates of bile acids, bile acid conjugates increases their detergent properties, prevents their precipitation in the acidic milieu of the upper small intestine, in intestines they are responsible for the solubilization and absorption of fats, vitamins and fat-soluble compounds CoA + taurocholate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.65 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.65 partial purification of recombinant enzyme, expressed in Escherichia coli DH5alpha Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.65 liver
-
 Mus musculus
-
2.3.1.65 additional information not in heart, brain, spleen, lung, skeletal muscle, kidney or testis tissue Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.65 choloyl-CoA + taurine taurine-specific enzyme, no use of glycine or fluoro-beta-alanine as substrates Mus musculus CoA + taurocholate
-
 ?
2.3.1.65 choloyl-CoA + taurine taurine-specific enzyme, gallbladder bile contains only taurine conjugates of bile acids, bile acid conjugates increases their detergent properties, prevents their precipitation in the acidic milieu of the upper small intestine, in intestines they are responsible for the solubilization and absorption of fats, vitamins and fat-soluble compounds Mus musculus CoA + taurocholate
-
 ?
2.3.1.65 additional information not: glycine, fluoro-beta-alanine Mus musculus ?
-
 ?
2.3.1.65 additional information Cys-234 may be involved in the formation of a bile acid thioester at the active site Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.65 ? x * 46525, calculated from the amino acid sequence, x * 45000, recombinant enzyme, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.65 mBAT
-
 Mus musculus