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Literature summary extracted from

  • Wu, D.; Hersh, L.B.
    Identification of an active site arginine in rat choline acetyltransferase by alanine scanning mutagenesis (1995), J. Biol. Chem., 270, 29111-29116.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.6 R452A kinetic as well chemical modification studies have implicated the presence of an active site arginine in enzyme, whose function is to stabilize coenzyme binding, conserved arginines are converted to identify these residues Rattus norvegicus
2.3.1.6 R453A kinetic as well chemical modification studies have implicated the presence of an active site arginine in enzyme, whose function is to stabilize coenzyme binding, conserved arginines are converted to identify these residues Rattus norvegicus
2.3.1.6 R458A kinetic as well chemical modification studies have implicated the presence of an active site arginine in enzyme, whose function is to stabilize coenzyme binding, conserved arginines are converted to identify these residues Rattus norvegicus
2.3.1.6 R463A kinetic as well chemical modification studies have implicated the presence of an active site arginine in enzyme, whose function is to stabilize coenzyme binding, conserved arginines are converted to identify these residues Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.6 0.00025
-
 CoA
-
 Rattus norvegicus
2.3.1.6 0.0004
-
 Acetylcholine
-
 Rattus norvegicus
2.3.1.6 0.0004
-
 CoA enzyme mutant R458A Rattus norvegicus
2.3.1.6 0.001
-
 CoA enzyme mutant R453A Rattus norvegicus
2.3.1.6 0.0011
-
 Acetylcholine enzyme mutant R463A Rattus norvegicus
2.3.1.6 0.0015
-
 Acetylcholine enzyme mutant R453A Rattus norvegicus
2.3.1.6 0.0016
-
 CoA enzyme mutant R463A Rattus norvegicus
2.3.1.6 0.002
-
 Acetylcholine enzyme mutant R458A Rattus norvegicus
2.3.1.6 0.0092
-
 Acetylcholine enzyme mutant R452A Rattus norvegicus
2.3.1.6 0.0154
-
 CoA enzyme mutant R452A Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.6 Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.6 acetyl-CoA + choline
-
 Rattus norvegicus acetylcholine + CoA
-
 ?