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Literature summary extracted from

  • Buckley, J.T.; Halasa, L.N.; MacIntyre, S.
    Purification and partial characterization of a bacterial phospholipid: cholesterol acyltransferase (1982), J. Biol. Chem., 257, 3320-3325.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.43 albumin stimulates Aeromonas salmonicida
2.3.1.43 apolipoprotein A-I
-
 Aeromonas salmonicida

General Stability

EC Number General Stability Organism
2.3.1.43 unaffected by repeated freezing and thawing Aeromonas salmonicida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.43 additional information no divalent cation requirement Aeromonas salmonicida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.43 23600
-
 x * 23600, SDS-PAGE Aeromonas salmonicida

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.43 Aeromonas salmonicida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.43 extraction from cell membranes with Tween 20, purification by Sephadex G-100 and DEAE-Sepharose columns Aeromonas salmonicida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.43 137
-
 in cholesterol-lecithin vesicles Aeromonas salmonicida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.43 additional information bacterial enzyme is not specific for phosphatidylcholine, it can use human erythrocyte membrane substrates, 2-positional specificity as acyltransferase Aeromonas salmonicida ?
-
 ?
2.3.1.43 additional information phospholipase A2 activity Aeromonas salmonicida ?
-
 ?
2.3.1.43 phosphatidylcholine + sterol
-
 Aeromonas salmonicida 1-acylglycerophosphocholine + sterol ester
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.43 ? x * 23600, SDS-PAGE Aeromonas salmonicida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.43 6 9 activity is independent of pH in this range Aeromonas salmonicida