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Literature summary extracted from

  • Marc, F.; Weigel, P.; Legrain, C.; Almeras, Y.; Santrot, M.; Glansdorff, N.; Sakanyan, V.
    Characterization and kinetic mechanism of mono- and bifunctional ornithine acetyltransferases from thermophilic microorganisms (2000), Eur. J. Biochem., 267, 5217-5226.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.35 expression in Escherichia coli Geobacillus stearothermophilus
2.3.1.35 expression in Escherichia coli Thermotoga neapolitana
2.3.1.35 expression in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.35 L-ornithine
-
 Geobacillus stearothermophilus
2.3.1.35 L-ornithine
-
 Methanocaldococcus jannaschii
2.3.1.35 L-ornithine
-
 Thermotoga neapolitana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.35 2.7
-
 alpha-N-acetyl-L-ornithine
-
 Geobacillus stearothermophilus
2.3.1.35 8.1
-
 alpha-N-acetyl-L-ornithine
-
 Thermotoga neapolitana
2.3.1.35 9.6
-
 alpha-N-acetyl-L-ornithine
-
 Methanocaldococcus jannaschii
2.3.1.35 11.3
-
 L-glutamate
-
 Methanocaldococcus jannaschii
2.3.1.35 19.2
-
 L-glutamate
-
 Geobacillus stearothermophilus
2.3.1.35 27.9
-
 L-glutamate
-
 Thermotoga neapolitana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate Geobacillus stearothermophilus first step in arginine biosynthesis L-ornithine + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate Thermotoga neapolitana first step in arginine biosynthesis L-ornithine + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate Methanocaldococcus jannaschii first step in arginine biosynthesis L-ornithine + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate Geobacillus stearothermophilus bifunctional enzyme catalyzes also the fifth step of arginine biosynthesis L-ornithine + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate Thermotoga neapolitana bifunctional enzyme catalyzes also the fifth step of arginine biosynthesis L-ornithine + N-acetyl-L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.35 Geobacillus stearothermophilus
-
-
-
2.3.1.35 Methanocaldococcus jannaschii
-
-
-
2.3.1.35 Thermotoga neapolitana
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.35 27
-
-
 Geobacillus stearothermophilus
2.3.1.35 175
-
-
 Methanocaldococcus jannaschii
2.3.1.35 290
-
-
 Thermotoga neapolitana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.35 acetyl-CoA + L-glutamate
-
 Geobacillus stearothermophilus CoA + N-acetyl-L-glutamate
-
 ?
2.3.1.35 acetyl-CoA + L-glutamate
-
 Thermotoga neapolitana CoA + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate
-
 Geobacillus stearothermophilus L-ornithine + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate
-
 Thermotoga neapolitana L-ornithine + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate
-
 Methanocaldococcus jannaschii L-ornithine + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate first step in arginine biosynthesis Geobacillus stearothermophilus L-ornithine + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate first step in arginine biosynthesis Thermotoga neapolitana L-ornithine + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate first step in arginine biosynthesis Methanocaldococcus jannaschii L-ornithine + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate bifunctional enzyme catalyzes also the fifth step of arginine biosynthesis Geobacillus stearothermophilus L-ornithine + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate bifunctional enzyme catalyzes also the fifth step of arginine biosynthesis Thermotoga neapolitana L-ornithine + N-acetyl-L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.35 heterotetramer
-
 Geobacillus stearothermophilus
2.3.1.35 heterotetramer
-
 Thermotoga neapolitana
2.3.1.35 heterotetramer
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.35 75
-
-
 Geobacillus stearothermophilus
2.3.1.35 90
-
-
 Thermotoga neapolitana
2.3.1.35 95
-
 more than 95°C Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.35 8
-
-
 Geobacillus stearothermophilus
2.3.1.35 8
-
-
 Thermotoga neapolitana
2.3.1.35 8
-
-
 Methanocaldococcus jannaschii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.35 7 10
-
 Geobacillus stearothermophilus
2.3.1.35 7 10
-
 Thermotoga neapolitana
2.3.1.35 7 10
-
 Methanocaldococcus jannaschii