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Literature summary extracted from

  • Shaw, W.V.; Brodsky, R.F.
    Characterization of chloramphenicol acetyltransferase from chloramphenicol-resistant Staphylococcus aureus (1968), J. Bacteriol., 95, 28-36.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.28 0.0027
-
 chloramphenicol
-
 Staphylococcus aureus
2.3.1.28 0.0037
-
 D-threo-1-p-nitrophenyl-2-bromoacetamido-1,3-propanediol
-
 Escherichia coli
2.3.1.28 0.0061
-
 chloramphenicol
-
 Escherichia coli
2.3.1.28 0.021
-
 D-threo-1-phenyl-2-dichloroacetamido-1,3-propanediol
-
 Escherichia coli
2.3.1.28 0.022
-
 D-threo-1-p-nitrophenyl-2-bromoacetamido-1,3-propanediol
-
 Staphylococcus aureus
2.3.1.28 0.068
-
 D-threo-1-phenyl-2-dichloroacetamido-1,3-propanediol
-
 Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.28 78000
-
 sucrose density gradient centrifugation Staphylococcus aureus
2.3.1.28 78000
-
 sucrose density gradient centrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.28 acetyl-CoA + chloramphenicol Staphylococcus aureus inducible enzyme CoA + chloramphenicol 3-acetate
-
 ?
2.3.1.28 acetyl-CoA + chloramphenicol Staphylococcus aureus inactivates chloramphenicol CoA + chloramphenicol 3-acetate
-
 ?
2.3.1.28 acetyl-CoA + chloramphenicol Escherichia coli inactivates chloramphenicol CoA + chloramphenicol 3-acetate
-
 ?
2.3.1.28 acetyl-CoA + chloramphenicol Staphylococcus aureus C22.1 inducible enzyme CoA + chloramphenicol 3-acetate
-
 ?
2.3.1.28 acetyl-CoA + chloramphenicol Staphylococcus aureus C22.1 inactivates chloramphenicol CoA + chloramphenicol 3-acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.28 Escherichia coli
-
-
-
2.3.1.28 Staphylococcus aureus
-
-
-
2.3.1.28 Staphylococcus aureus C22.1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.28 2 acetyl-CoA + chloramphenicol
-
 Staphylococcus aureus 2 CoA + chloramphenicol 1,3-diacetate
-
 ?
2.3.1.28 acetyl-CoA + chloramphenicol
-
 Escherichia coli CoA + chloramphenicol 3-acetate
-
 ?
2.3.1.28 acetyl-CoA + chloramphenicol acetylates only the biologically active D-threo stereoisomer Staphylococcus aureus CoA + chloramphenicol 3-acetate + chloramphenicol 1,3-diacetate ?
2.3.1.28 acetyl-CoA + chloramphenicol inducible enzyme Staphylococcus aureus CoA + chloramphenicol 3-acetate
-
 ?
2.3.1.28 acetyl-CoA + chloramphenicol inactivates chloramphenicol Staphylococcus aureus CoA + chloramphenicol 3-acetate
-
 ?
2.3.1.28 acetyl-CoA + chloramphenicol inactivates chloramphenicol Escherichia coli CoA + chloramphenicol 3-acetate
-
 ?
2.3.1.28 acetyl-CoA + chloramphenicol acetylates only the biologically active D-threo stereoisomer Staphylococcus aureus C22.1 CoA + chloramphenicol 3-acetate + chloramphenicol 1,3-diacetate ?
2.3.1.28 acetyl-CoA + chloramphenicol inducible enzyme Staphylococcus aureus C22.1 CoA + chloramphenicol 3-acetate
-
 ?
2.3.1.28 acetyl-CoA + chloramphenicol inactivates chloramphenicol Staphylococcus aureus C22.1 CoA + chloramphenicol 3-acetate
-
 ?
2.3.1.28 acetyl-CoA + D-threo-1-p-nitrophenyl-2-bromoacetamido-1,3-propanediol
-
 Staphylococcus aureus CoA + ?
-
 ?
2.3.1.28 acetyl-CoA + D-threo-1-p-nitrophenyl-2-bromoacetamido-1,3-propanediol
-
 Escherichia coli CoA + ?
-
 ?
2.3.1.28 acetyl-CoA + D-threo-1-p-nitrophenyl-2-bromoacetamido-1,3-propanediol
-
 Staphylococcus aureus C22.1 CoA + ?
-
 ?
2.3.1.28 acetyl-CoA + D-threo-1-phenyl-2-dichloroacetamido-1,3-propanediol
-
 Staphylococcus aureus CoA + ?
-
 ?
2.3.1.28 acetyl-CoA + D-threo-1-phenyl-2-dichloroacetamido-1,3-propanediol
-
 Escherichia coli CoA + ?
-
 ?
2.3.1.28 acetyl-CoA + D-threo-1-phenyl-2-dichloroacetamido-1,3-propanediol
-
 Staphylococcus aureus C22.1 CoA + ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.28 75
-
 rapid inactivation Escherichia coli
2.3.1.28 75
-
 remarkably resistant Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.28 7.8
-
-
 Staphylococcus aureus
2.3.1.28 7.8
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.28 6 8.8 pH 6.0: about 55% of maximal activity, pH 8.8: about 85% of maximal activity Staphylococcus aureus