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Literature summary extracted from

  • Johnson, T.M.; Mann, W.R.; Dragland, C.J.; Anderson, R.C.; Nemecek, G.M.; Bell, P.A.
    Over-expression and characterization of active recombinant rat liver carnitine palmitoyltransferase II using baculovirus (1995), Biochem. J., 309, 689-693.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.21 cloning and expression of CPT II in Escherichia coli, overexpression of CPT II in Spodoptera frugiperda Sf 9 cells via baculavirus infection, DNA sequence analysis Rattus norvegicus

General Stability

EC Number General Stability Organism
2.3.1.21 no loss in activity after 6 freeze/thaw cycles at -70°C or room temeprature, recombinant CPT II Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.21 0.034
-
 palmitoyl-CoA recombinant CPT II Rattus norvegicus
2.3.1.21 0.95
-
 L-carnitine recombinant CPT II Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.21 acyl-CoA + L-carnitine Rattus norvegicus
-
 CoA + L-acylcarnitine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.21 Rattus norvegicus
-
 enzyme form CPT II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.21 recombinant CPT II from Sf 9 insect cells Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.21 liver CPT II Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.21 0.43
-
 recombinant CPT II from Sf 9 insect cells Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.3.1.21 4°C, pH 7.0-10.0, 24 h stable, recombinant CPT II Rattus norvegicus
2.3.1.21 room temperature, 6.5 h, no loss in activity, recombinant CPT II Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.21 acyl-CoA + L-carnitine
-
 Rattus norvegicus CoA + L-acylcarnitine
-
 ?
2.3.1.21 palmitoyl-CoA + L-carnitine
-
 Rattus norvegicus CoA + L-palmitoylcarnitine
-
 ?

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.21 7 10 stable for 24 h at 4°C, recombinant CPT II Rattus norvegicus