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Literature summary extracted from

  • Thompson, D.K.; Chen, J.C.
    Purification and properties of an acetoacetyl coenzyme A-reacting phosphotransbutyrylase from Clostridium beijerinckii (Clostridium butylicum) NRRL B593 (1990), Appl. Environ. Microbiol., 56, 607-613.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.19 0.04
-
butanoyl-CoA
-
Clostridium beijerinckii
2.3.1.19 1.1
-
acetoacetyl-CoA
-
Clostridium beijerinckii
2.3.1.19 3.33
-
acetyl-CoA
-
Clostridium beijerinckii
2.3.1.19 6.5
-
phosphate cosubstrate butyryl-CoA Clostridium beijerinckii
2.3.1.19 12.9
-
phosphate cosubstrate acetoacetyl-CoA Clostridium beijerinckii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.19 33000
-
x * 33000, SDS-PAGE Clostridium beijerinckii
2.3.1.19 205000
-
gel filtration Clostridium beijerinckii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.19 butanoyl-CoA + phosphate Clostridium beijerinckii role in energy metabolism in butyric acid bacteria butanoylphosphate + CoA
-
?
2.3.1.19 butanoyl-CoA + phosphate Clostridium beijerinckii VPI 13437 role in energy metabolism in butyric acid bacteria butanoylphosphate + CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.19 Clostridium beijerinckii
-
identical with Clostridium butylicum NRRL B593
-
2.3.1.19 Clostridium beijerinckii VPI 13437
-
identical with Clostridium butylicum NRRL B593
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.19
-
Clostridium beijerinckii

Storage Stability

EC Number Storage Stability Organism
2.3.1.19 -20°C, less purified preparation, 50% v/v glycerol, 50% loss of activity in 5 d Clostridium beijerinckii
2.3.1.19 liquid N2, purified preparation, 50 mM sodium phosphate buffer, pH 7.0, various NaCl concentrations, 92-100% loss of activity Clostridium beijerinckii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.19 acetoacetyl-CoA + phosphate
-
Clostridium beijerinckii acetoacetylphosphate + CoA
-
r
2.3.1.19 acetoacetyl-CoA + phosphate
-
Clostridium beijerinckii VPI 13437 acetoacetylphosphate + CoA
-
r
2.3.1.19 acetyl-CoA + phosphate
-
Clostridium beijerinckii acetylphosphate + CoA
-
?
2.3.1.19 acetyl-CoA + phosphate
-
Clostridium beijerinckii VPI 13437 acetylphosphate + CoA
-
?
2.3.1.19 butanoyl-CoA + phosphate
-
Clostridium beijerinckii butanoylphosphate + CoA
-
?
2.3.1.19 butanoyl-CoA + phosphate role in energy metabolism in butyric acid bacteria Clostridium beijerinckii butanoylphosphate + CoA
-
?
2.3.1.19 butanoyl-CoA + phosphate
-
Clostridium beijerinckii VPI 13437 butanoylphosphate + CoA
-
?
2.3.1.19 butanoyl-CoA + phosphate role in energy metabolism in butyric acid bacteria Clostridium beijerinckii VPI 13437 butanoylphosphate + CoA
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.19 ? x * 33000, SDS-PAGE Clostridium beijerinckii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.19 7.5
-
-
Clostridium beijerinckii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.19 6.5 8 butanoylphosphate formation, 50% of maximal activity at pH 6.5, 80% of maximal activity at pH 8.0 Clostridium beijerinckii