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Literature summary extracted from

  • Baumert, A.; Maier, W.; Gröger, D.; Deutzmann, R.
    Purification and properties of acridone synthase from cell suspension cultures of Ruta graveolens L. (1994), Z. Naturforsch. C, 49, 26-32.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.159 iodoacetamide 0.5 mM: 95% inhibition Ruta graveolens
2.3.1.159 N-ethylmaleimide 1 mM: 100% inhibition Ruta graveolens
2.3.1.159 p-chloromercuribenzoate 0.5 mM: 95% inhibition Ruta graveolens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.159 0.0106
-
 N-methylanthraniloyl-CoA partially purified enzyme Ruta graveolens
2.3.1.159 0.0328
-
 malonyl-CoA partially purified enzyme Ruta graveolens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.159 additional information
-
 amino acid sequence determination Ruta graveolens
2.3.1.159 40000
-
 2 * 40000, SDS-PAGE Ruta graveolens
2.3.1.159 69000
-
 gel filtration Ruta graveolens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.159 malonyl-CoA + N-methylanthraniloyl-CoA Ruta graveolens
-
 CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.159 Ruta graveolens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.159
-
 Ruta graveolens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.159 cell suspension culture
-
 Ruta graveolens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.159 6.035
-
 purified enzyme Ruta graveolens

Storage Stability

EC Number Storage Stability Organism
2.3.1.159 -70°C, partially purified, stable for at least 4 weeks Ruta graveolens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.159 malonyl-CoA + N-methylanthraniloyl-CoA
-
 Ruta graveolens CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.159 dimer 2 * 40000, SDS-PAGE Ruta graveolens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.159 32
-
 assay at Ruta graveolens