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Literature summary extracted from

  • Fritz, P.K.; Kauffman, J.M.; Robertson, C.A.; Wilson, M.R.
    Cocoa butter biosynthesis. Purification and characterization of a soluble sn-glycerol-3-phosphate acyltransferase from cocoa seeds (1986), J. Biol. Chem., 261, 194-199.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.15 Antibodies to cocoa seed enzyme raised in rabbit Theobroma cacao

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.15 0.004
-
 stearoyl-CoA
-
 Theobroma cacao
2.3.1.15 4.2
-
 sn-glycerol 3-phosphate
-
 Theobroma cacao

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 cytoplasm
-
 Theobroma cacao 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.15 20000
-
 10 * 20000, SDS-PAGE Theobroma cacao
2.3.1.15 200000
-
 gel filtration Theobroma cacao

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Theobroma cacao initial reaction of cocoa butter biosynthesis CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Theobroma cacao
-
 cocoa
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15 ammonium sulfate, DEAE-cellulose, Sephacryl S-300 Theobroma cacao

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.15 cotyledon
-
 Theobroma cacao
-
2.3.1.15 seed
-
 Theobroma cacao
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.15 0.69
-
-
 Theobroma cacao

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate exclusively acylates glycerol 3-phosphate at the C-1-position Theobroma cacao CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate initial reaction of cocoa butter biosynthesis Theobroma cacao CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate
-
 Theobroma cacao CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 stearoyl-CoA + sn-glycerol 3-phosphate
-
 Theobroma cacao CoA + 1-stearoyl-sn-glycerol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.15 decamer 10 * 20000, SDS-PAGE Theobroma cacao