EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.133 | EDTA | no effect | Raphanus sativus | |
2.3.1.133 | EDTA | no effect | Cichorium endivia |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.99 | Ca2+ | inhibition at 5 mM | Cichorium endivia | |
2.3.1.99 | DTT | concentration above 5 mM results in a marked inhibition of activity | Cichorium endivia | |
2.3.1.99 | Mg2+ | inhibition at 5 mM | Cichorium endivia | |
2.3.1.133 | Ca2+ | inhibition at 5 mM | Cichorium endivia | |
2.3.1.133 | Ca2+ | inhibition at 5 mM | Raphanus sativus | |
2.3.1.133 | DTT | concentration above 5 mM results in a marked inhibition of activity | Cichorium endivia | |
2.3.1.133 | DTT | concentration above 5 mM results in a marked inhibition of activity | Raphanus sativus | |
2.3.1.133 | Mg2+ | inhibition at 5 mM | Cichorium endivia | |
2.3.1.133 | Mg2+ | inhibition at 5 mM | Raphanus sativus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.99 | 10 | - |
coumaroyl-CoA | - |
Cichorium endivia | |
2.3.1.99 | 11.4 | - |
feruloyl-CoA | - |
Cichorium endivia | |
2.3.1.99 | 14.3 | - |
caffeoyl-CoA | - |
Cichorium endivia | |
2.3.1.133 | 12.5 | - |
caffeoyl-CoA | - |
Cichorium endivia | |
2.3.1.133 | 14.3 | - |
4-coumaroyl-CoA | - |
Raphanus sativus | |
2.3.1.133 | 14.3 | - |
4-coumaroyl-CoA | - |
Cichorium endivia | |
2.3.1.133 | 15.4 | - |
feruloyl-CoA | - |
Cichorium endivia | |
2.3.1.133 | 30.5 | - |
caffeoyl-CoA | - |
Raphanus sativus | |
2.3.1.133 | 55.5 | - |
feruloyl-CoA | - |
Raphanus sativus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.133 | Ca2+ | sligthly stimulated by 3.0 mM, inhibition at 5 mM | Raphanus sativus | |
2.3.1.133 | Ca2+ | sligthly stimulated by 3.0 mM, inhibition at 5 mM | Cichorium endivia | |
2.3.1.133 | Mg2+ | slightly stimulated by 0.5 mM, inhibition at 5 mM | Raphanus sativus | |
2.3.1.133 | Mg2+ | slightly stimulated by 0.5 mM, inhibition at 5 mM | Cichorium endivia |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.99 | 40000 | - |
gel filtration | Cichorium endivia |
2.3.1.133 | 40000 | - |
gel filtration | Raphanus sativus |
2.3.1.133 | 40000 | - |
gel filtration | Cichorium endivia |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.133 | 4-coumaroyl-CoA + shikimate | Raphanus sativus | hydroxycinnamic acid-shikimate ester biosynthesis | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
2.3.1.133 | 4-coumaroyl-CoA + shikimate | Cichorium endivia | hydroxycinnamic acid-shikimate ester biosynthesis | CoA + 5-O-(4-coumaroyl)shikimate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.99 | Cichorium endivia | - |
- |
- |
2.3.1.133 | Cichorium endivia | - |
- |
- |
2.3.1.133 | Raphanus sativus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.99 | partial | Cichorium endivia |
2.3.1.133 | - |
Cichorium endivia |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.1.99 | leaf | - |
Cichorium endivia | - |
2.3.1.133 | leaf | - |
Raphanus sativus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.133 | additional information | - |
- |
Cichorium endivia |
EC Number | Storage Stability | Organism |
---|---|---|
2.3.1.99 | -20°C, for several months, no apparent loss of activity | Cichorium endivia |
2.3.1.133 | -20°C, for several months, no apparent loss of activity | Cichorium endivia |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.99 | 4-coumaroyl-CoA + quinate | - |
Cichorium endivia | CoA + 5-O-(4-coumaroyl)quinate | - |
? | |
2.3.1.99 | caffeoyl-CoA + quinate | - |
Cichorium endivia | CoA + 5-O-caffeoylquinate | - |
? | |
2.3.1.99 | feruloyl-CoA + quinate | - |
Cichorium endivia | CoA + O-feruloylquinate | - |
? | |
2.3.1.133 | 4-coumaroyl-CoA + quinate | - |
Raphanus sativus | CoA + 4-coumaroylquinate | - |
? | |
2.3.1.133 | 4-coumaroyl-CoA + shikimate | - |
Raphanus sativus | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
2.3.1.133 | 4-coumaroyl-CoA + shikimate | - |
Cichorium endivia | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
2.3.1.133 | 4-coumaroyl-CoA + shikimate | hydroxycinnamic acid-shikimate ester biosynthesis | Raphanus sativus | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
2.3.1.133 | 4-coumaroyl-CoA + shikimate | hydroxycinnamic acid-shikimate ester biosynthesis | Cichorium endivia | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
2.3.1.133 | caffeoyl-CoA + shikimate | - |
Raphanus sativus | CoA + 5-O-caffeoylshikimate | - |
? | |
2.3.1.133 | caffeoyl-CoA + shikimate | - |
Cichorium endivia | CoA + 5-O-caffeoylshikimate | - |
? | |
2.3.1.133 | feruloyl-CoA + shikimate | - |
Raphanus sativus | CoA + 5-O-feruloylshikimate | - |
? | |
2.3.1.133 | feruloyl-CoA + shikimate | - |
Cichorium endivia | CoA + 5-O-feruloylshikimate | - |
? | |
2.3.1.133 | additional information | no substrate: cinnamoyl-CoA, L-malate, D(+)-malate, L(+)-tartrate, m-tartrate, citrate, glucose, UDP-glucose, myo-inositol, tyramine, agmatine | Raphanus sativus | ? | - |
? | |
2.3.1.133 | additional information | no substrate: cinnamoyl-CoA, L-malate, D(+)-malate, L(+)-tartrate, m-tartrate, citrate, glucose, UDP-glucose, myo-inositol, tyramine, agmatine | Cichorium endivia | ? | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.99 | additional information | - |
pI: 5.1 | Cichorium endivia |
2.3.1.99 | 6.5 | - |
50 mM potassium phosphate buffer | Cichorium endivia |
2.3.1.133 | 6.5 | - |
50 mM potassium phosphate buffer | Cichorium endivia |
2.3.1.133 | 6.5 | - |
pI: 4.33 | Cichorium endivia |
2.3.1.133 | 6.8 | - |
50 mM potassium phosphate buffer | Raphanus sativus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.133 | 5 | 9 | - |
Raphanus sativus |
2.3.1.133 | 5 | 9 | - |
Cichorium endivia |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.3.1.99 | Cichorium endivia | - |
- |
5.1 |