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Literature summary extracted from

  • Tollbom, Í.; Valtersson, C.; Chojnacki, T.; Dallner, G.
    Esterification of dolichol in rat liver (1988), J. Biol. Chem., 263, 1347-1352.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.123 albumin activates Rattus norvegicus
2.3.1.123 Triton X-100 activates at low concentrations by 50% Rattus norvegicus

General Stability

EC Number General Stability Organism
2.3.1.123 Triton X-100 stabilizes Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.123 beta-octylglycopyranoside strong, almost complete inactivation at 0.04% Rattus norvegicus
2.3.1.123 Ca2+ 20 mM, 50-60% inhibition Rattus norvegicus
2.3.1.123 deoxycholate strong, almost complete inactivation at 0.04% Rattus norvegicus
2.3.1.123 Mn2+ 20 mM, 50-60% inhibition Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.123 microsome liver, esterification takes place on cytoplasmic side of membranes of the endoplasmic reticulum and is subsequently distributed to different organelles Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.123 additional information KCl and MgCl2 have no influence Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.123 acetyl-CoA + dolichol Rattus norvegicus
-
 CoA + dolichyl acetate
-
 ?
2.3.1.123 arachidonoyl-CoA + dolichol Rattus norvegicus
-
 CoA + dolichyl arachidonate
-
 ?
2.3.1.123 additional information Rattus norvegicus esterfication may play a role in targeting the lipid from the endoplasmic reticulum to lysosomes ?
-
 ?
2.3.1.123 myristoyl-CoA + dolichol Rattus norvegicus
-
 CoA + dolichyl myristate
-
 ?
2.3.1.123 oleoyl-CoA + dolichol Rattus norvegicus
-
 CoA + dolichyl oleate
-
 ?
2.3.1.123 palmitoyl-CoA + dolichol Rattus norvegicus
-
 CoA + dolichyl palmitate
-
 ?
2.3.1.123 stearoyl-CoA + dolichol Rattus norvegicus
-
 CoA + dolichyl stearate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.123 Rattus norvegicus
-
 male
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.123 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.123 brain slight activity Rattus norvegicus
-
2.3.1.123 heart slight activity Rattus norvegicus
-
2.3.1.123 kidney slight activity Rattus norvegicus
-
2.3.1.123 liver
-
 Rattus norvegicus
-
2.3.1.123 lung slight activity Rattus norvegicus
-
2.3.1.123 muscle slight activity Rattus norvegicus
-
2.3.1.123 small intestine slight activity Rattus norvegicus
-
2.3.1.123 spleen slight activity Rattus norvegicus
-
2.3.1.123 thymus slight activity Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.3.1.123 -20°C, microsomes are stable for several months Rattus norvegicus
2.3.1.123 4°C, microsomes in 0.25 M sucrose, after 5 days 50% remaining activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.123 acetyl-CoA + dolichol
-
 Rattus norvegicus CoA + dolichyl acetate
-
 ?
2.3.1.123 acetyl-CoA + dolichol 30% of activity compared to palmitoyl-CoA Rattus norvegicus CoA + dolichyl acetate
-
 ?
2.3.1.123 arachidonoyl-CoA + dolichol
-
 Rattus norvegicus CoA + dolichyl arachidonate
-
 ?
2.3.1.123 arachidonoyl-CoA + dolichol 30% of activity compared to palmitoyl-CoA Rattus norvegicus CoA + dolichyl arachidonate
-
 ?
2.3.1.123 additional information alpha-saturated dolichols are acylated more rapidly than alpha-unsaturated analogues Rattus norvegicus ?
-
 ?
2.3.1.123 additional information esterfication may play a role in targeting the lipid from the endoplasmic reticulum to lysosomes Rattus norvegicus ?
-
 ?
2.3.1.123 myristoyl-CoA + dolichol
-
 Rattus norvegicus CoA + dolichyl myristate
-
 ?
2.3.1.123 myristoyl-CoA + dolichol 40% of activity compared to palmitoyl-CoA Rattus norvegicus CoA + dolichyl myristate
-
 ?
2.3.1.123 oleoyl-CoA + dolichol
-
 Rattus norvegicus CoA + dolichyl oleate
-
 ?
2.3.1.123 oleoyl-CoA + dolichol 40% of activity compared to palmitoyl-CoA Rattus norvegicus CoA + dolichyl oleate
-
 ?
2.3.1.123 palmitoyl-CoA + dolichol
-
 Rattus norvegicus CoA + dolichyl palmitate
-
 ?
2.3.1.123 stearoyl-CoA + dolichol
-
 Rattus norvegicus CoA + dolichyl stearate
-
 ?
2.3.1.123 stearoyl-CoA + dolichol 40% of activity compared to palmitoyl-CoA Rattus norvegicus CoA + dolichyl stearate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.123 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.123 6.4
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.123 5 7 20% of maximal activity at pH 5.0, 40% of maximal activity at pH 7.0 Rattus norvegicus