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Literature summary extracted from

  • Butterworth, P.J.; Tsai, S.; Eley, M.H.; Roche, T.E.; Reed, L.J.
    A kinetic study of dihydrolipoyl transacetylase from bovine kidney (1975), J. Biol. Chem., 250, 1921-1925.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.12 1,2-diselenolane-3-valeric acid competitive with respect to dihydrolipoamide and non-competitive with respect to acetyl-CoA Bos taurus
2.3.1.12 CoA competitive to acetyl-CoA, non-competitive to dihydrolipoamide Bos taurus
2.3.1.12 lipoamidase complete loss of activity after 3 h Bos taurus
2.3.1.12 palmitoyl-CoA competitive to acetyl-CoA, non-competitive to dihydrolipoamide Bos taurus
2.3.1.12 S-acetyldihydrolipoamide competitive to dihydrolipoamide, non-competitive to acetyl-CoA Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.12 0.03 0.1 acetyl-CoA
-
 Bos taurus
2.3.1.12 0.13 0.4 dihydrolipoamide
-
 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.12 dihydrolipoamide + acetyl-CoA Bos taurus acetyl group is transferred from the S-acetyldihydrolipoyl moiety bound covalently to the transacetylase to CoA S-acetyldihydrolipoamide + CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.12 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.12 lipoprotein
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.12 acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine = CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine reaction mechanism Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.12 kidney
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.12 dihydrolipoamide + acetyl-CoA
-
 Bos taurus S-acetyldihydrolipoamide + CoA
-
 r
2.3.1.12 dihydrolipoamide + acetyl-CoA acetyl group is transferred from the S-acetyldihydrolipoyl moiety bound covalently to the transacetylase to CoA Bos taurus S-acetyldihydrolipoamide + CoA
-
 r