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Literature summary extracted from

  • Hein, D.; Doll, M.; Rustan, T.; Ferguson, R.
    Metabolic activation of N-hydroxyarylamines and N-hydroxyarylamides by 16 recombinant human NAT2 allozymes: effects of 7 specific NAT2 nucleic acid substitutions (1995), Cancer Res., 55, 3531-3536.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.56 expression of wild-type and mutants in Escherichia coli strains DH5alpha and JM105 Homo sapiens
2.3.1.118 16 recombinant NAT2 allozymes expressed in Escherichia coli JM105 expression system Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.56 G286E small reduction in activity Homo sapiens
2.3.1.56 I114T strong reduction of the acetylation activity Homo sapiens
2.3.1.56 K268R slightly increased activity Homo sapiens
2.3.1.56 additional information nucleic acid substitutions C282T and C481T have no effect on activity Homo sapiens
2.3.1.56 R197Q moderate reduction of acetylation activity Homo sapiens
2.3.1.56 R64Q moderate reduction of acetylation activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.118 paraoxon no significant effect Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.56 cytosol
-
 Homo sapiens 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.118 33500
-
 SDS PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.56 Homo sapiens
-
-
-
2.3.1.118 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.56 colon
-
 Homo sapiens
-
2.3.1.118 colon
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.56 additional information
-
 N-, O-, and N,O-acetyltransferase activity, wild-type and mutants Homo sapiens
2.3.1.118 additional information
-
 radiolabel assay and Eadie-Hofstee plot Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.56 N-hydroxy-2-acetylaminofluorene + N-hydroxy-4-aminobiphenyl
-
 Homo sapiens N-hydroxy-2-aminofluorene + N-acetoxy-4-aminobiphenyl
-
 r
2.3.1.56 N-hydroxy-4-acetylaminobiphenyl + N-hydroxy-4-aminobiphenyl
-
 Homo sapiens N-hydroxy-4-aminobiphenyl + N-acetoxy-4-aminobiphenyl
-
 r
2.3.1.118 acetyl-CoA + 2-aminofluorene N-acetyltransferase activity Homo sapiens CoA + 2-acetylaminofluorene
-
 ?
2.3.1.118 acetyl-CoA + N-hydroxy-2-aminofluorene
-
 Homo sapiens CoA + N-acetoxy-2-aminofluorene
-
 ?
2.3.1.118 acetyl-CoA + N-hydroxy-4-aminobiphenyl
-
 Homo sapiens CoA + N-acetoxy-4-aminobiphenyl
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.56 37
-
 assay at Homo sapiens
2.3.1.118 37
-
 N-acetyltransferase assay Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.118 7.4
-
 O-acetyltransferase assay Homo sapiens